PEOPLE

Bruce L. Levine, PhD

Barbara and Edward Netter Professor in Cancer Gene Therapy
University of Pennsylvania Perelman School of Medicine

Contact InformationCenter for Cellular Immunotherapies
Smilow 8-114
3400 Civic Center Boulevard

Philadelphia, PA, 19104
Office: 215-746-1481

Email: levinebl@pennmedicine.upenn.edu

Specialty Division

Transfusion Medicine & Therapeutic Pathology

Research Expertise

Immunology, Cell and Gene Therapy

Other Expertise

Good Manufacturing Practices

Itmat Expertise

Dr. Levine's interests include cell, gene, and biologic therapy clinical trial development, manufacturing, and testing and lymphocyte growth, activation and senescence.

Education

B.A. (Major-Biology, Minor-History), The University of Pennsylvania , 1984
Ph.D. (Immunology and Infectious Diseases), The Johns Hopkins University, 1992

Specialty Certification

Postgraduate Training

Post-doctoral Fellow, Henry M. Jackson Foundation, Naval Medical Research Institute, National Naval Medical Center, Bethesda, MD, 1992-1996

Awards and Honors

Honorable Mention, The Frederik Bang Award for Student Research, 1991
Sustained Superior Performance Award, 1997. The Henry M. Jackson Foundation, 1995
New Investigator Award, Clinical Immunology Society, 1997
Dynal Literature Award for 1996, 1997
Best Abstract Award International Society for Cellular Therapy Annual Meeting, 2004
Best Abstract Award International Society for Cellular Therapy Annual Meeting, 2007
Discover Magazine Top 100 Stories of 2011: #2 Designer Cells Block HIV, http://discovermagazine.com/2012/jan-feb/02; #10 Leukemia Cure, http://discovermagazine.com/2012/jan-feb/10, 2011
Highest Rated Scientific Abstract (blinded review), International Society for Cellular Therapy 17th Annual Meeting, 2011
Science Translational Medicine Top Downloaded paper for 2011, "T cells with chimerical antigen receptors have potent antitumor effects and can establish memory in patients with advanced leukemia", 2012
Dr. Audrey E. Evans Award of Excellence, Philadelphia Ronald McDonald House, 2014
Clinical Research Achievement Award from the Clinical Research Forum for Gene editing of CCR5 in autologous CD4 T cells of persons infected with HIV, 2015
Best Oral Abstract Award, International Society for Cellular Therapy Annual Meeting, 2015
Best Oral Abstract Award, International Society for Cellular Therapy Annual Meeting, 2016
William Osler Patient Oriented Research Award from the University of Pennsylvania Perelman School of Medicine, 2018

Memberships and Professional Organizations

American Society of Gene Therapy, 1999 - Present
International Society for Cellular Therapy, 1999 - present
American Association for the Advancement of Science, 2002 - Present
Abstract reviewer for International Society for Cellular Therapy Annual Meeting, 2003 - Present
NIH Study Section, Immunology Integrated Review Group SSS-4 SBIR/STTR, 2004 - Present
Abstract reviewer for American Society for Gene Therapy Annual Meeting, 2004 - Present
Abstract reviewer for International Society for Cellular Therapy Annual Meeting, Gene Therapy, 2004 - Present
Abstract reviewer for International Society for Cellular Therapy Annual Meeting, Gene Therapy, 2005 - Present
NIH/NIAID Study Section, Center for AIDS Research: D-CFAR, CFAR, 2005 - Present
Abstract reviewer for International Society for Cellular Therapy Annual Meeting, Gene Therapy, 2006 - Present
NIH/NIAID Clinical Trial Planning (R34) Grants Study Section, 2006 - Present
Abstract reviewer for American Society for Gene Therapy Annual Meeting, Cell Processing and Vector Production, 2007 - Present
Abstract reviewer for International Society for Cellular Therapy Annual Meeting, Gene Therapy, 2007 - Present
Coordinating Abstract reviewer for American Society for Gene Therapy Annual Meeting, Cell Processing and Vector Production, 2008 - Present
Abstract reviewer for International Society for Cellular Therapy Annual Meeting, Gene Therapy, 2008 - Present
NIH/NIAID Study Section, Center for AIDS Research: D-CFAR, CFAR, 2009 - Present
Cambridge Healthtech Institute, 2010 - Present
Abstract reviewer for American Society for Gene Therapy Annual Meeting, Cell Processing and Vector Production, 2010 - Present
NIH/NHLBI Program: Production Assistance for Cellular Therapies, 2010 - Present
European Commission review panel for FP7 Cooperation Work Programme "HEALTH.2010.1.4-1: Translational research on cell-based immunotherapy" Brussels, Belgium, 2010 - 2011
American Society for Gene and Cell Therapy, 2011 - Present
European Commission review panel for FP7 Cooperation Work Programme "HEALTH.2012.1.4-4: "Targeted nucleic acid delivery as an innovative therapeutic or prophylactic approach" Brussels, Belgium, 2011 - 2012
Cancer Prevention and Research Institute of Texas: Texas Access to Cancer Cell Therapies (TACCT), 2014 - Present

Web Links


Selected Publications

Induction of resistance to chimeric antigen receptor T cell therapy by transduction of a single leukemic B cell.

Ruella M, Xu J, Barrett DM, Fraietta JA, Reich TJ, Ambrose DE, Klichinsky M, Shestova O, Patel PR, Kulikovskaya I, Nazimuddin F, Bhoj VG, Orlando EJ, Fry TJ, Bitter H, Maude SL, Levine BL, Nobles CL, Bushman FD, Young RM, Scholler J, Gill SI, June CH, Grupp SA, Lacey SF, Melenhorst JJ, Nature Medicine 24(10): 1499-1503, 2018

Reducing Ex Vivo Culture Improves the Anti-leukemic Activity of Chimeric Antigen Receptor (CAR)-T Cells

Ghassemi S, Nunez-Cruz S, O'Connor RS, Fraietta JA, Patel PR, Scholler J, Barrett DM, Lundh SM, Davis MM, Bedoya F, Leferovich J, Lacey SF, Levine BL, Grupp SA, June CH, Melenhorst JJ, Milone MC, Cancer Immunol Res. 6(9): 100-1109, 2018

Clinical Pharmacology of Tisagenlecleucel in B-Cell Acute Lymphoblastic Leukemia.

Mueller Karen T, Waldron Edward R, Grupp Stephan A, Levine John E, Laetsch Theodore W, Pulsipher Michael A, Boyer Michael W, August Keith, Hamilton Jason, Awasthi Rakesh, Stein Andrew M, Sickert Denise, Chakraborty Abhijit, Levine Bruce L, June Carl H, Tomassian Lori, Shah Sweta, Leung Mimi, Taran Tetiana, Wood Patricia A, Maude Shannon L, Clinical Cancer Research, 2018, PMID:30190371

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Non-viral RNA chimeric antigen receptor modified T cells in patients with Hodgkin lymphoma

Svoboda J, Rheingold SR, Gill SI, Grupp SA, Lacey SF, Kulikovskaya I, Suhoski MM, Melenhorst JJ, Loudon B, Mato AR, Nasta SD, Landsburg DJ, Youngman MR, Levine BL, Porter DL, June CH, Schuster SJ, Blood 132(10): 1022-1026, 2018

Disruption of TET2 promotes the therapeutic efficacy of CD19-targeted T cells

Fraietta JA1, Nobles CL, Sammons MA, Lundh S, Carty SA, Reich TJ, Cogdill AP, Morrissette JJD, DeNizio JE, Reddy S, Hwang Y, Gohil M, Kulikovskaya I, Nazimuddin F, Gupta M, Chen F, Everett JK, Alexander KA, Lin-Shiao E, Gee MH, Liu X, Young RM, Ambrose D, Wang Y, Xu J, Jordan MS, Marcucci KT, Levine BL, Garcia KC, Zhao Y, Kalos M, Porter DL, Kohli RM, Lacey SF, Berger SL, Bushman FD, June CH, Melenhorst JJ, Nature 558(7709): 307-312, 2018

Determinants of response and resistance to CD19 chimeric antigen receptor (CAR) T cell therapy of chronic lymphocytic leukemia.

Fraietta Joseph A, Lacey Simon F, Orlando Elena J, Pruteanu-Malinici Iulian, Gohil Mercy, Lundh Stefan, Boesteanu Alina C, Wang Yan, O'Connor Roddy S, Hwang Wei-Ting, Pequignot Edward, Ambrose David E, Zhang Changfeng, Wilcox Nicholas, Bedoya Felipe, Dorfmeier Corin, Chen Fang, Tian Lifeng, Parakandi Harit, Gupta Minnal, Young Regina M, Johnson F Brad, Kulikovskaya Irina, Liu Li, Xu Jun, Kassim Sadik H, Davis Megan M, Levine Bruce L, Frey Noelle V, Siegel Donald L, Huang Alexander C, Wherry E John, Bitter Hans, Brogdon Jennifer L, Porter David L, June Carl H, Melenhorst J Joseph, Nature medicine 24(5): 563-571, 2018, PMID:29713085

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Personalized cancer vaccine effectively mobilizes antitumor T cell immunity in ovarian cancer.

Tanyi Janos L, Bobisse Sara, Ophir Eran, Tuyaerts Sandra, Roberti Annalisa, Genolet Raphael, Baumgartner Petra, Stevenson Brian J, Iseli Christian, Dangaj Denarda, Czerniecki Brian, Semilietof Aikaterini, Racle Julien, Michel Alexandra, Xenarios Ioannis, Chiang Cheryl, Monos Dimitri S, Torigian Drew A, Nisenbaum Harvey L, Michielin Olivier, June Carl H, Levine Bruce L, Powell Daniel J, Gfeller David, Mick Rosemarie, Dafni Urania, Zoete Vincent, Harari Alexandre, Coukos George, Kandalaft Lana E, Science translational medicine 10(436): eaao5931, 2018, PMID:29643231

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Anti-CD19 CAR T cells with high-dose melphalan and autologous stem cell transplantation for refractory multiple myeloma.

Garfall Alfred L, Stadtmauer Edward A, Hwang Wei-Ting, Lacey Simon F, Melenhorst Jan Joseph, Krevvata Maria, Carroll Martin P, Matsui William H, Wang Qiuju, Dhodapkar Madhav V, Dhodapkar Kavita, Das Rituparna, Vogl Dan T, Weiss Brendan M, Cohen Adam D, Mangan Patricia A, Ayers Emily C, Nunez-Cruz Selene, Kulikovskaya Irina, Davis Megan M, Lamontagne Anne, Dengel Karen, Kerr Naseem Ds, Young Regina M, Siegel Donald L, Levine Bruce L, Milone Michael C, Maus Marcela V, June Carl H, JCI insight 3(8): 120505, 2018, PMID:29669947

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Activity of Mesothelin-specific Chimeric Antigen Receptor T cells Against Pancreatic Carcinoma Metastases in a Phase 1 Trial.

Beatty Gregory L, O'Hara Mark H, Lacey Simon F, Torigian Drew A, Nazimuddin Farzana, Chen Fang, Kulikovskaya Irina M, Soulen Michael C, McGarvey Maureen, Nelson Anne Marie, Gladney Whitney L, Levine Bruce L, Melenhorst J Joseph, Plesa Gabriela, June Carl H, Gastroenterology, 2018, PMID:29567081

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Tisagenlecleucel in Children and Young Adults with B-Cell Lymphoblastic Leukemia.

Maude Shannon L, Laetsch Theodore W, Buechner Jochen, Rives Susana, Boyer Michael, Bittencourt Henrique, Bader Peter, Verneris Michael R, Stefanski Heather E, Myers Gary D, Qayed Muna, De Moerloose Barbara, Hiramatsu Hidefumi, Schlis Krysta, Davis Kara L, Martin Paul L, Nemecek Eneida R, Yanik Gregory A, Peters Christina, Baruchel Andre, Boissel Nicolas, Mechinaud Francoise, Balduzzi Adriana, Krueger Joerg, June Carl H, Levine Bruce L, Wood Patricia, Taran Tetiana, Leung Mimi, Mueller Karen T, Zhang Yiyun, Sen Kapildeb, Lebwohl David, Pulsipher Michael A, Grupp Stephan A, The New England journal of medicine 378(5): 439-448, 2018, PMID:29385370

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