PEOPLE

Michael C. Milone, MD, PhD

Associate Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Associate Director, Toxicology Laboratory
University of Pennsylvania Perelman School of Medicine

Contact Information3400 Spruce Street 7103 Founders Pavilion
Philadelphia, PA 19104
Office: 215-662-6575
Fax: 215-662-7529

Email: milone@pennmedicine.upenn.edu

Specialty Division

Laboratory Medicine, Immunobiology and Experimental Pathology

Research Expertise

Research in my laboratory is focused on T cell biology and applications of genetically-engineered T cells to the treatment of disease. Active research projects in my laboratory include:

1) Developing chimeric antigen receptors (CARs) for adoptive immunotherapy of cancer with enhanced function and improved safety.

2) Developing and applying synthetic immunoreceptors to the treatment of immune-mediated disease.

3) Exploring the role of co-stimulatory signals in directing T cell metabolism and the way this metabolism affects T cell function within tumors.

 

Clinical Expertise

Clinical Laboratory Toxicology, Therapeutic Drug Monitoring, Clinical Chemistry, Blood Banking/Transfusion Medicine, Therapeutic Apheresis

Itmat Expertise

My laboratory is focused upon developing methods to use the immune system to treat cancer. Active research includes 1) exploring the mechanical properties of artificial adhesion substrates used in T cell culture and their influence on T cell biology, 2) development of artificial antigen receptors and signaling systems for control of T cell specificity

Graduate Groups

Cell and Molecular Biology

Education

B.S (Chemical Biology), Stevens Institute of Technology, 1993
Ph.D (Experimental Pathology/ Immunology), UMDNJ - Graduate School of Biomedical Sciences, 1999
M.D. (Medical Doctor), UMDNJ- New Jersey Medical School, 1999

Specialty Certification

Clinical Pathology, American Board of Pathology, 2002

Postgraduate Training

Internship, Internal Medicine, Hospital of the University of Pennsylvania, 1999-2000
Resident, Clinical Pathology, Hospital of the University of Pennsylvania, 2000-2002
Chief Resident, Division of Laboratory Medicine, Hospital of the University of Pennsylvania, 2001-2002
Clinical Fellowship, Blood Banking and Transfusion Medicine, Hospital of the University of Pennsylvania, 2002-2003
Postdoctoral. Research Fellow, Translational Research Program, Abramson Family Cancer Institute, University of Pennsylvania School of Medicine, 2003-2007
Clinical Fellow, Clinical Chemistry and Laboratory Toxicology, Hospital of the University of Pennsylvania, 2004-2007

Awards and Honors

Stanley S. Bergen Award for Academic Excellence, UMDNJ, 1996
Faculty Organization Award, UMDNJ-New Jersey Medical School, 1999
Stanely S. Bergen Medal of Excellence, UMDNJ, 1999
The Peter C. Nowell Teaching Award, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 2011
Top Ten Clinical Research Achievement Award, Clinical Research Forum, 2017

Memberships and Professional Organizations

American Society of Clinical Pharmacology and Therapeutics, 2007 - 2010
American Association of Clinical Chemistry, 2007 - Present
College of American Pathologists, 2008 - Present
American Society of Hematology, 2008 - Present
Academy of Clinical Laboratory Physicians and Scientists, 2008 - 2015
American Association of Immunologists, 2009 - Present
International Association of Therapeutic Drug Monitoring and Clinical Toxicology, 2009 - Present
American Society of Gene and Cell Therapy, 2009 - Present
American Society of Clinical Pathology, 2015 - Present
National Institute for innovation in Manufacturing Biopharmaceuticals (NIIMBL), 2017 - Present

Web Links


Selected Publications

Reducing Ex Vivo Culture Improves the Anti-leukemic Activity of Chimeric Antigen Receptor (CAR)-T Cells.

Ghassemi Saba, Nunez-Cruz Selene, O'Connor Roddy S, Fraietta Joseph A, Patel Prachi R, Scholler John, Barrett David M, Lundh Stefan M, Davis Megan M, Bedoya Felipe, Leferovich John, Lacey Simon F, Levine Bruce L, Grupp Stephan A, June Carl H, Melenhorst J Joseph, Milone Michael C, Cancer immunology research, 2018, PMID:30030295

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Clinical use of lentiviral vectors.

Milone Michael C, O'Doherty Una, Leukemia 32(7): 1529-1541, 2018, PMID:29654266

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False-positive results following lentiviral-based tisagenlecleucel therapy with select HIV-1 NAT methods.

Laetsch Theodore W, Maude Shannon L, Milone Michael C, Davis Kara L, Krueger Joerg, Cardenas Ana Maria, Eldjerou Lamis K, Keir Christopher H, Wood Patricia A, Grupp Stephan A, Blood 131(23): 2596-2598, 2018, PMID:29669777

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Pre-clinical validation of B cell maturation antigen (BCMA) as a target for T cell immunotherapy of multiple myeloma

De-xiu Bu, Reshma Singh, Eugene E. Choi,*, Marco Ruella, Selene Nunez- Cruz, Keith G. Mansfi eld, Paul Bennett, Nathanial Barton, Qilong Wu, Jiquan Zhang, Yongqiang Wang, Lai Wei, Shawn Cogan, Tucker Ezell, Shree Joshi, Kellie J. Latimer, Brian Granda, William R. Tschantz, Regina M. Young, Heather A. Huet, Celeste J. Richardson and Michael C. Milone, Oncotarget 9(40): 25764-25780, 2018, PMID:29899820

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The CPT1a inhibitor, etomoxir induces severe oxidative stress at commonly used concentrations.

O'Connor Roddy S, Guo Lili, Ghassemi Saba, Snyder Nathaniel W, Worth Andrew J, Weng Liwei, Kam Yoonseok, Philipson Benjamin, Trefely Sophie, Nunez-Cruz Selene, Blair Ian A, June Carl H, Milone Michael C, Scientific reports 8(1): 6289, 2018, PMID:29674640

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Neurotoxicity Associated with a High-Affinity GD2 CAR-Response.

Richman Sarah A, Milone Michael C, Cancer immunology research 6(4): 496-497, 2018, PMID:29610424

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Anti-CD19 CAR T cells with high-dose melphalan and autologous stem cell transplantation for refractory multiple myeloma.

Garfall Alfred L, Stadtmauer Edward A, Hwang Wei-Ting, Lacey Simon F, Melenhorst Jan Joseph, Krevvata Maria, Carroll Martin P, Matsui William H, Wang Qiuju, Dhodapkar Madhav V, Dhodapkar Kavita, Das Rituparna, Vogl Dan T, Weiss Brendan M, Cohen Adam D, Mangan Patricia A, Ayers Emily C, Nunez-Cruz Selene, Kulikovskaya Irina, Davis Megan M, Lamontagne Anne, Dengel Karen, Kerr Naseem Ds, Young Regina M, Siegel Donald L, Levine Bruce L, Milone Michael C, Maus Marcela V, June Carl H, JCI insight 3(8): , 2018, PMID:29669947

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High-Affinity GD2-Specific CAR T Cells Induce Fatal Encephalitis in a Preclinical Neuroblastoma Model.

Richman Sarah A, Nunez-Cruz Selene, Moghimi Babak, Li Lucy Z, Gershenson Zachary T, Mourelatos Zissimos, Barrett David M, Grupp Stephan A, Milone Michael C, Cancer immunology research 6(1): 36-46, 2018, PMID:29180536

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Powered and controlled T-cell production

Michael C. Milone and Bruce L. Levine, Nature Biomedical Engineering 2(): 148-150, 2018

The pharmacology of T cell therapies

Vijay Bhoj and Michael C. Milone, Molecular Therapy - Methods & Clinical Development 8(): 210-221, 2018