Michael C. Milone, MD, PhD
Associate Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Associate Director, Toxicology Laboratory
University of Pennsylvania Perelman School of Medicine
Contact Information3400 Spruce Street 7103 Founders Pavilion
Philadelphia, PA 19104
Office: 215-662-6575
Fax: 215-662-7529
Email: milone@pennmedicine.upenn.edu
Specialty Division
Laboratory Medicine, Immunobiology and Experimental Pathology
Research Expertise
Research in my laboratory is focused on T cell biology and applications of genetically-engineered T cells to the treatment of disease. Active research projects in my laboratory include:
1) Developing chimeric antigen receptors (CARs) for adoptive immunotherapy of cancer with enhanced function and improved safety.
2) Developing and applying synthetic immunoreceptors to the treatment of immune-mediated disease.
3) Exploring the role of co-stimulatory signals in directing T cell metabolism and the way this metabolism affects T cell function within tumors.
Clinical Expertise
Clinical Laboratory Toxicology, Therapeutic Drug Monitoring, Clinical Chemistry, Blood Banking/Transfusion Medicine, Therapeutic Apheresis
Itmat Expertise
My laboratory is focused upon developing methods to use the immune system to treat cancer. Active research includes 1) exploring the mechanical properties of artificial adhesion substrates used in T cell culture and their influence on T cell biology, 2) development of artificial antigen receptors and signaling systems for control of T cell specificity
Graduate Groups
Cell and Molecular Biology
Education
B.S (Chemical Biology), Stevens Institute of Technology, 1993
Ph.D (Experimental Pathology/ Immunology), UMDNJ - Graduate School of Biomedical Sciences, 1999
M.D. (Medical Doctor), UMDNJ- New Jersey Medical School, 1999
Specialty Certification
Clinical Pathology, American Board of Pathology, 2002
Postgraduate Training
Internship, Internal Medicine, Hospital of the University of Pennsylvania, 1999-2000
Resident, Clinical Pathology, Hospital of the University of Pennsylvania, 2000-2002
Chief Resident, Division of Laboratory Medicine, Hospital of the University of Pennsylvania, 2001-2002
Clinical Fellowship, Blood Banking and Transfusion Medicine, Hospital of the University of Pennsylvania, 2002-2003
Postdoctoral. Research Fellow, Translational Research Program, Abramson Family Cancer Institute, University of Pennsylvania School of Medicine, 2003-2007
Clinical Fellow, Clinical Chemistry and Laboratory Toxicology, Hospital of the University of Pennsylvania, 2004-2007
Awards and Honors
Stanley S. Bergen Award for Academic Excellence, UMDNJ, 1996
Faculty Organization Award, UMDNJ-New Jersey Medical School, 1999
Stanely S. Bergen Medal of Excellence, UMDNJ, 1999
The Peter C. Nowell Teaching Award, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 2011
Top Ten Clinical Research Achievement Award, Clinical Research Forum, 2017
Top 20 Clinical Research Papers for 2019, Clinical Research Forum, 2019
Memberships and Professional Organizations
American Society of Clinical Pharmacology and Therapeutics, 2007 - 2010
American Association of Clinical Chemistry, 2007 - Present
Academy of Clinical Laboratory Physicians and Scientists, 2008 - 2015
College of American Pathologists, 2008 - Present
American Society of Hematology, 2008 - Present
American Society of Gene and Cell Therapy, 2009 - Present
American Association of Immunologists, 2009 - Present
International Association of Therapeutic Drug Monitoring and Clinical Toxicology, 2009 - Present
American Society of Clinical Pathology, 2015 - Present
National Institute for innovation in Manufacturing Biopharmaceuticals (NIIMBL), 2017 - Present
Web Links
Selected Publications
Ligand-Induced Degradation of a CAR Permits Reversible Remote Control of CAR T Cell Activity In Vitro and In Vivo
Richman, S. A., Wang, L. C., Khire, U. R., Albelda, S. M., Milone, M. C., Mol Ther, 2020, PMID:32559430
4-1BB costimulation promotes CAR T cell survival through noncanonical NF-κB signaling.
Philipson Benjamin I, O'Connor Roddy S, May Michael J, June Carl H, Albelda Steven M, Milone Michael C, Science signaling 13(625): , 2020, PMID:32234960
Imaging CAR T Cell Trafficking with eDHFR as a PET Reporter Gene.
Sellmyer Mark A, Richman Sarah A, Lohith Katheryn, Hou Catherine, Weng Chi-Chang, Mach Robert H, O'Connor Roddy S, Milone Michael C, Farwell Michael D, Molecular therapy : the journal of the American Society of Gene Therapy 28(1): 42-51, 2020, PMID:31668558
Testing the Specificity of Compounds Designed to Inhibit CPT1A in T Cells.
O'Connor Roddy S, Milone Michael C, Methods in molecular biology (Clifton, N.J.) 2097(): 83-90, 2020, PMID:31776920
Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy.
Ghassemi Saba, Milone Michael C, Journal of visualized experiments : JoVE, 2019, PMID:31904011
Commentary.
Sheng Feng, Milone Michael C, Clinical chemistry 65(11): 1361, 2019, PMID:31662335
T-cell phenotypes associated with effective CAR T-cell therapy in postinduction vs relapsed multiple myeloma.
Garfall Alfred L, Dancy Ehren K, Cohen Adam D, Hwang Wei-Ting, Fraietta Joseph A, Davis Megan M, Levine Bruce L, Siegel Don L, Stadtmauer Edward A, Vogl Dan T, Waxman Adam, Rapoport Aaron P, Milone Michael C, June Carl H, Melenhorst J Joseph, Blood advances 3(19): 2812-2815, 2019, PMID:31575532
An introduction to chimeric antigen receptor (CAR) T-cell immunotherapy for human cancer.
Feins Steven, Kong Weimin, Williams Erik F, Milone Michael C, Fraietta Joseph A, American journal of hematology 94(S1): S3-S9, 2019, PMID:30680780
Immunotherapy Using Chimeric Antigen Receptor-Engineered T Cells: A Novel Cellular Therapy with Important Implications for the Clinical Laboratory.
Thibodeaux Suzanne R, Milone Michael C, Clinical chemistry 65(4): 519-529, 2019, PMID:30593467
B cell maturation antigen-specific CAR T cells are clinically active in multiple myeloma.
Cohen Adam D, Garfall Alfred L, Stadtmauer Edward A, Melenhorst J Joseph, Lacey Simon F, Lancaster Eric, Vogl Dan T, Weiss Brendan M, Dengel Karen, Nelson Annemarie, Plesa Gabriela, Chen Fang, Davis Megan M, Hwang Wei-Ting, Young Regina M, Brogdon Jennifer L, Isaacs Randi, Pruteanu-Malinici Iulian, Siegel Don L, Levine Bruce L, June Carl H, Milone Michael C, The Journal of clinical investigation 130(): 2210-2221, 2019, PMID:30896447
