Michael C. Milone, MD, PhD

Associate Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Associate Director, Toxicology Laboratory
University of Pennsylvania Perelman School of Medicine

Contact Information3400 Spruce Street 7103 Founders Pavilion
Philadelphia, PA 19104
Office: 215-662-6575
Fax: 215-662-7529


Specialty Division

Laboratory Medicine, Immunobiology and Experimental Pathology

Research Expertise

Research in my laboratory is focused on T cell biology and applications of genetically-engineered T cells to the treatment of disease. Active research projects in my laboratory include:

1) Developing chimeric antigen receptors (CARs) for adoptive immunotherapy of cancer with enhanced function and improved safety.

2) Developing and applying synthetic immunoreceptors to the treatment of immune-mediated disease.

3) Exploring the role of co-stimulatory signals in directing T cell metabolism and the way this metabolism affects T cell function within tumors.

Clinical Expertise

Clinical Laboratory Toxicology, Therapeutic Drug Monitoring, Clinical Chemistry, Blood Banking/Transfusion Medicine, Therapeutic Apheresis

Itmat Expertise

My laboratory is focused upon developing methods to use the immune system to treat cancer. Active research includes 1) exploring the mechanical properties of artificial adhesion substrates used in T cell culture and their influence on T cell biology, 2) development of artificial antigen receptors and signaling systems for control of T cell specificity

Graduate Groups

Cell and Molecular Biology


B.S (Chemical Biology), Stevens Institute of Technology, 1993
Ph.D (Experimental Pathology/ Immunology), UMDNJ - Graduate School of Biomedical Sciences, 1999
M.D. (Medical Doctor), UMDNJ- New Jersey Medical School, 1999

Specialty Certification

Clinical Pathology, American Board of Pathology, 2002

Postgraduate Training

Internship, Internal Medicine, Hospital of the University of Pennsylvania, 1999-2000
Resident, Clinical Pathology, Hospital of the University of Pennsylvania, 2000-2002
Chief Resident, Division of Laboratory Medicine, Hospital of the University of Pennsylvania, 2001-2002
Clinical Fellowship, Blood Banking and Transfusion Medicine, Hospital of the University of Pennsylvania, 2002-2003
Postdoctoral. Research Fellow, Translational Research Program, Abramson Family Cancer Institute, University of Pennsylvania School of Medicine, 2003-2007
Clinical Fellow, Clinical Chemistry and Laboratory Toxicology, Hospital of the University of Pennsylvania, 2004-2007

Awards and Honors

Stanley S. Bergen Award for Academic Excellence, UMDNJ, 1996
Stanely S. Bergen Medal of Excellence, UMDNJ, 1999
Faculty Organization Award, UMDNJ-New Jersey Medical School, 1999
The Peter C. Nowell Teaching Award, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 2011
Top Ten Clinical Research Achievement Award, Clinical Research Forum, 2017

Memberships and Professional Organizations

American Association of Clinical Chemistry, 2007 - Present
American Society of Clinical Pharmacology and Therapeutics, 2007 - 2010
College of American Pathologists, 2008 - Present
Academy of Clinical Laboratory Physicians and Scientists, 2008 - 2015
American Society of Hematology, 2008 - Present
International Association of Therapeutic Drug Monitoring and Clinical Toxicology, 2009 - Present
American Society of Gene and Cell Therapy, 2009 - Present
American Association of Immunologists, 2009 - Present
American Society of Clinical Pathology, 2015 - Present

Web Links

Selected Publications

Results of ASERTAA, a Randomized Prospective Crossover Pharmacogenetic Study of Immediate-Release Versus Extended-Release Tacrolimus in African American Kidney Transplant Recipients.

Trofe-Clark Jennifer, Brennan Daniel C, West-Thielke Patricia, Milone Michael C, Lim Mary Ann, Neubauer Robin, Nigro Vincenza, Bloom Roy D, American journal of kidney diseases : the official journal of the National Kidney Foundation, 2017, PMID:29162334

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High-Affinity GD2-Specific CAR T Cells Induce Fatal Encephalitis in a Preclinical Neuroblastoma Model.

Richman Sarah A, Nunez-Cruz Selene, Moghimi Babak, Li Lucy Z, Gershenson Zachary T, Mourelatos Zissimos, Barrett David M, Grupp Stephan A, Milone Michael C, Cancer immunology research, 2017, PMID:29180536

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Improving T Cell Expansion with a Soft Touch.

Lambert Lester H, Goebrecht Geraldine K E, De Leo Sarah E, O'Connor Roddy S, Nunez-Cruz Selene, Li Tai-De, Yuan Jinglun, Milone Michael C, Kam Lance C, Nano letters 17(2): 821-826, 2017, PMID:28122453

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Programmed death ligand-1 expression on donor T cells drives graft-versus-host disease lethality.

Saha Asim, O'Connor Roddy S, Thangavelu Govindarajan, Lovitch Scott B, Dandamudi Durga Bhavani, Wilson Caleph B, Vincent Benjamin G, Tkachev Victor, Pawlicki Jan M, Furlan Scott N, Kean Leslie S, Aoyama Kazutoshi, Taylor Patricia A, Panoskaltsis-Mortari Angela, Foncea Rocio, Ranganathan Parvathi, Devine Steven M, Burrill Joel S, Guo Lili, Sacristan Catarina, Snyder Nathaniel W, Blair Ian A, Milone Michael C, Dustin Michael L, Riley James L, Bernlohr David A, Murphy William J, Fife Brian T, Munn David H, Miller Jeffrey S, Serody Jonathan S, Freeman Gordon J, Sharpe Arlene H, Turka Laurence A, Blazar Bruce R, The Journal of clinical investigation 126(7): 2642-60, 2016, PMID:27294527

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Reengineering chimeric antigen receptor T cells for targeted therapy of autoimmune disease.

Ellebrecht Christoph T, Bhoj Vijay G, Nace Arben, Choi Eun Jung, Mao Xuming, Cho Michael Jeffrey, Di Zenzo Giovanni, Lanzavecchia Antonio, Seykora John T, Cotsarelis George, Milone Michael C*, Payne Aimee S*, Science (New York, N.Y.) 353(6295): 179-84, 2016, PMID:27365313

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Persistence of long-lived plasma cells and humoral immunity in individuals responding to CD19-directed CAR T-cell therapy.

Bhoj Vijay G, Arhontoulis Dimitrios, Wertheim Gerald, Capobianchi James, Callahan Colleen A, Ellebrecht Christoph T, Obstfeld Amrom E, Lacey Simon F, Melenhorst Jan J, Nazimuddin Farzana, Hwang Wei-Ting, Maude Shannon L, Wasik Mariusz A, Bagg Adam, Schuster Stephen, Feldman Michael D, Porter David L, Grupp Stephen A, June Carl H, Milone Michael C, Blood 128(3): 360-70, 2016, PMID:27166358

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High-Throughput Mechanobiology Screening Platform Using Micro- and Nanotopography.

Hu Junqiang, Gondarenko Alexander A, Dang Alex P, Bashour Keenan T, O'Connor Roddy S, Lee Sunwoo, Liapis Anastasia, Ghassemi Saba, Milone Michael C, Sheetz Michael P, Dustin Michael L, Kam Lance C, Hone James C, Nano letters In Press(4): 2198-204, 2016, PMID:26990380

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Distinct Signaling of Coreceptors Regulates Specific Metabolism Pathways and Impacts Memory Development in CAR T Cells.

Kawalekar Omkar U, O'Connor Roddy S, Fraietta Joseph A, Guo Lili, McGettigan Shannon E, Posey Avery D, Patel Prachi R, Guedan Sonia, Scholler John, Keith Brian, Snyder Nathaniel, Blair Ian, Milone Michael C*, June Carl H*, Immunity 44(2): 380-90, 2016, PMID:26885860

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The Addition of the BTK inhibitor Ibrutinib to Anti-CD19 Chimeric Antigen Receptor T Cells (CART19) Improves Responses against Mantle Cell Lymphoma.

Ruella Marco, Kenderian Saad S, Shestova Olga, Fraietta Joseph A, Qayyum Sohail, Zhang Qian, Maus Marcela V, Liu Xiaobin, Nunez-Cruz Selene, Klichinsky Michael, Kawalekar Omkar U, Lacey Simon F, Milone Michael, Mato Anthony R, Schuster Stephen J, Kalos Michael, June Carl H, Gill Saar, Wasik Mariusz A, Clin Cancer Res 22(11): 2684-96, 2016, PMID:26819453

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Chimeric antigen receptor T cells persist and induce sustained remissions in relapsed refractory chronic lymphocytic leukemia.

Porter DL, Hwang W-T, Frey NV, Lacey SF, Shaw PA, Loren AW, Bagg A, Marcucci KT, Shen A, Gonzalez V, Ambrose D, Grupp SA, Chew A, Zheng Z, Milone MC, Levine BL, Melenhorst JJ, June CH, Sci Transl Med 7(303): 303ra139, 2015, PMID:26333935

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