Guilherme P.F. Nader, PhD
Assistant Professor of Pathology and Laboratory Medicine
Assistant Professor of Cell and Developmental Biology
Perelman School of Medicine at the University of Pennsylvania
Contact InformationChildren's Hospital of Philadelphia
Department of Pathology and Laboratory Medicine
Abramson Pediatric Research Center, Rm. 816D
3615 Civic Center Blvd
Philadelphia, PA 19104
Tel: 267-425-2124
Email: naderg@chop.edu
Research Expertise
Our research program focuses on elucidating how the cell nucleus affects cell function and fate as a consequence of different degrees of nuclear deformation observed during cell migration in complex microenvironments and tissue crowding.
Keywords: nuclear mechanosensing, DNA damage, cell migration, nuclear deformation, microfabrication.
For more details: www.naderlab.org
Education
BS University of São Paulo, Ribeirão Preto, Brazil, 2006
MS University of São Paulo, Ribeirão Preto, Brazil, 2009
PhD Columbia University, New York, 2015
Specialty Certification
Postgraduate Training
Postdoctoral Research Scientist, Institut Curie/Institut Pierre-Gilles de Gennes, Paris, 2016-22
Awards and Honors
National Council of Scientific and Technology Development Scholarship for Undergraduate Student Research, Brazil, 2006-07
Fellowship for Masters Student Research (FAPESP), Brazil, 2007-09
Best Poster Award for poster entitled “An integrin endocytic recycling pathway mediated by FAK and Src controls the polarized reassembly of focal adhesions after their disassembly,” Integrated Program Retreat, Columbia University, 2012
ASCB Travel Award for the ASCB Annual Meeting in San Francisco, CA, USA, 2012
ASCB Travel Award for the ASCB Annual Meeting in New Orleans, LA, USA, 2013
ASCB Travel Award for the ASCB Annual Meeting in Washington, DC, USA, 2019
Grandes Avancées Françaises en Biologie 2022 – Prize awarded by Institut de France-Académie des sciences, 2022
Memberships and Professional Organizations
American Society for Cell Biology (ASCB), 2013-present
Web Links
Selected Publications
The kinesin KIF3AC recycles endocytosed integrin to polarize new adhesion formation toward the leading edge
Rockenbach JAZ, Nader GPF, Antoku S, Gundersen GG. Proc Natl Acad Sci U S A 122(30):e2513776122, Jul 2025. doi: 10.1073/pnas.2513776122. Epub 2025 Jul 24. PMID: 40705420; PMCID: PMC12318216.
Chromosome mis-segregation triggers cell cycle arrest through a mechanosensitive nuclear envelope checkpoint
Hervé S, Scelfo A, Bersano Marchisio G, Grison M, Vaidžiulytė K, Dumont M, Angrisani A, Keikhosravi A, Pegoraro G, Deygas M, P F Nader G, Macé AS, Gentili M, Williart A, Manel N, Piel M, Miroshnikova YA, Fachinetti D. Nat Cell Biol 27(1):73-86, Jan 2025. doi: 10.1038/s41556-024-01565-x. Epub 2025 Jan 8. PMID: 39779939; PMCID: PMC11735390.
Cell shape sensing licenses dendritic cells for homeostatic migration to lymph nodes
Alraies Z, Rivera CA, Delgado MG, Sanséau D, Maurin M, Amadio R, Maria Piperno G, Dunsmore G, Yatim A, Lacerda Mariano L, Kniazeva A, Calmettes V, Sáez PJ, Williart A, Popard H, Gratia M, Lamiable O, Moreau A, Fusilier Z, Crestey L, Albaud B, Legoix P, Dejean AS, Le Dorze AL, Nakano H, Cook DN, Lawrence T, Manel N, Benvenuti F, Ginhoux F, Moreau HD, P F Nader G, Piel M, Lennon-Duménil AM. Nat Immunol 25(7):1193-1206, Jul 2024. doi: 10.1038/s41590-024-01856-3. Epub 2024 Jun 4. PMID: 38834865; PMCID: PMC11224020.
DNA damage induces nuclear envelope rupture through ATR-mediated phosphorylation of Lamin A/C
Kovacs MT, Vallette M, Wiertsema P, Dingli F, Loew D, Nader GPF, Piel M, Ceccaldi R. Mol Cell 83(20):3659-3668.e10, Oct 2023. doi: 10.1016/j.molcel.2023.09.023. Epub 2023 Oct 12. PMID: 37832547; PMCID: PMC10597398.
Nuclear envelope disruption triggers hallmarks of aging in lung alveolar macrophages
De Silva NS, Siewiera J, Alkhoury C, Nader GPF, Nadalin F, de Azevedo K, Couty M, Izquierdo HM, Bhargava A, Conrad C, Maurin M, Antoniadou K, Fouillade C, Londono-Vallejo A, Behrendt R, Bertotti K, Serdjebi C, Lanthiez F, Gallwitz L, Saftig P, Herrero-Fernández B, Saez A, González-Granado JM, van Niel G, Boissonnas A, Piel M, Manel N. Nat Aging 3(10):1251-1268, Oct 2023. doi: 10.1038/s43587-023-00488-w. Epub 2023 Sep 18. PMID: 37723209.
Tissue fluidification promotes a cGAS-STING cytosolic DNA response in invasive breast cancer
Frittoli E, Palamidessi A, Iannelli F, Zanardi F, Villa S, Barzaghi L, Abdo H, Cancila V, Beznoussenko GV, Della Chiara G, Pagani M, Malinverno C, Bhattacharya D, Pisati F, Yu W, Galimberti V, Bonizzi G, Martini E, Mironov AA, Gioia U, Ascione F, Li Q, Havas K, Magni S, Lavagnino Z, Pennacchio FA, Maiuri P, Caponi S, Mattarelli M, Martino S, d'Adda di Fagagna F, Rossi C, Lucioni M, Tancredi R, Pedrazzoli P, Vecchione A, Petrini C, Ferrari F, Lanzuolo C, Bertalot G, Nader G, Foiani M, Piel M, Cerbino R, Giavazzi F, Tripodo C, Scita G. Nat Mater 22(5):644-655, May 2023. doi: 10.1038/s41563-022-01431-x. Epub 2022 Dec 29. Erratum in: Nat Mater. 2023 Mar;22(3):400. doi: 10.1038/s41563-023-01479-3. PMID: 36581770; PMCID: PMC10156599.
Nuclear fragility, blaming the Blebs
Srivastava N, Nader GPF*, Williart A, Rollin R, Cuvelier D, Lomakin A, Piel M. Curr Opin Cell Biol 70:100-108, Jun 2021. doi: 10.1016/j.ceb.2021.01.007. Epub 2021 Mar 1. PMID: 33662810. *Co-first author
Compromised nuclear envelope integrity drives TREX1-dependent DNA damage and tumor cell invasion
Nader GPF, Agüera-Gonzalez S, Routet F, Gratia M, Maurin M, Cancila V, Cadart C, Palamidessi A, Ramos RN, San Roman M, Gentili M, Yamada A, Williart A, Lodillinsky C, Lagoutte E, Villard C, Viovy JL, Tripodo C, Galon J, Scita G, Manel N, Chavrier P, Piel M. Cell 184(20):5230-5246.e22, Sep 2021. doi: 10.1016/j.cell.2021.08.035. Epub 2021 Sep 21. PMID: 34551315.
Nuclear deformations, from signaling to perturbation and damage
Nader GPF, Williart A, Piel M. Curr Opin Cell Biol 72:137-145, Oct 2021. doi: 10.1016/j.ceb.2021.07.008. Epub 2021 Aug 27. PMID: 34461580.
The nucleus acts as a ruler tailoring cell responses to spatial constraints
Lomakin AJ, Cattin CJ, Cuvelier D, Alraies Z, Molina M, Nader GPF, Srivastava N, Sáez PJ, Garcia-Arcos JM, Zhitnyak IY, Bhargava A, Driscoll MK, Welf ES, Fiolka R, Petrie RJ, De Silva NS, González-Granado JM, Manel N, Lennon-Duménil AM, Müller DJ, Piel M. Science 370(6514):eaba2894, Oct 2020. doi: 10.1126/science.aba2894. PMID: 33060332; PMCID: PMC8059074.
