Mohamad-Gabriel Alameh, PhD
Assistant Professor of Pathology and Laboratory MedicinePerelman School of Medicine at the University of Pennsylvania
Contact InformationDepartment of Pathology and Laboratory Medicine
Colket Translational Research Building (CTRB)
Office 11-018
3501 Civic Center Blvd
Philadelphia, PA 19104
Tel: 215-590-1815
Email: mg.alameh@pennmedicine.upenn.edu; alamehm@chop.edu
Specialty Division
Cancer and Immunobiology, CHOP Experimental Pathology, CHOP Laboratory Medicine
Research Expertise
Non-viral drug delivery system including Lipid and polymer based nanoparticles, adjuvant activity of ionizable lipids, formulation science, process development and optimization, mRNA deigns and production (small and large scale), mRNA based vaccines for bacterial diseases, and in utero gene delivery
Graduate Groups
Biochemistry and Molecular Biophysics
Bioengineering
Immunology
Education
BSc (Microbiology and Immunology), University of Montreal, 2006
MSc (Theoretical Molecular Biology), University of Montreal, 2009
PhD (Biomedical Engineering, Nanotechnology and Nanomedicine), Polytechnique Montreal, 2017
Specialty Certification
Postgraduate Training
Postdoctoral Fellow, mRNA vaccines, University of Pennsylvania, 2018-23
Awards and Honors
The Fakhry Alameh Foundation Scholarship for Academic Excellence, 2006
TechniScience Student Leadership Award, 2007
University of Montreal and Paul Sabatier Award for the Development of Medicines, 2014
The Hariri Foundation Scholarship, 2015
Duke Consortia for HIV/AIDS Vaccine Development (CHAVD), 2023
AbuDhabi Global Innovation Award, 2025
Memberships and Professional Organizations
American Society of Cell and Gene Therapy, 2011-present
The Controlled Release Society, 2011-present
Canadian Chapter of the Society of Controlled Release, 2014-present
New York Academy of Science, 2018-19
The Richard Society, 2024
RNA Canada, 2024
Web Links
Selected Publications
Patient-Specific In Vivo Gene Editing to Treat a Rare Genetic Disease
Musunuru K, Grandinette SA, Wang X, Hudson TR, Briseno K, ...Barbosa CJ, Semple SC, Tam YK, Lutz C, George LA, Kleinstiver BP, Liu DR, Ng K, Kassim SH, Giannikopoulos P, Alameh MG, Urnov FD, Ahrens-Nicklas RC. N Engl J Med 392(22):2235-2243, Jun 2025. doi: 10.1056/NEJMoa2504747. Epub 2025 May 15. PMID: 40373211.
Comparative Analysis of Chitosan, Lipid Nanoparticles, and Alum Adjuvants in Recombinant SARS-CoV-2 Vaccine: An Evaluation of Their Immunogenicity and Serological Efficacy
Ghattas M, Dwivedi G, Chevrier A, Scobey T, El-Mayta R, Mattocks MD, Wang D, Lavertu M*, Alameh MG.* Vaccines (Basel) 13(8):788, Jul 2025. doi: 10.3390/vaccines13080788. PMID: 40872875; PMCID: PMC12390328. *co-corresponding authors
Monitoring mRNA vaccine antigen expression in vivo using PET/CT
Blizard GS, Dwivedi G, Pan YG, Hou C, Etersque JM, Said H, Chevrier A, Lavertu M, Ni H, Davis B, Tam Y, Cao Q, Mach RH, Weissman D, Alameh MG*, Sellmyer MA.* Nat Commun 16(1):2234, Mar 2025. doi: 10.1038/s41467-025-57446-w. PMID: 40044669; PMCID: PMC11882883. *co-senior and co-corresponding authors
A multivalent mRNA-LNP vaccine protects against Clostridioides difficile infection
Alameh MG*, Semon A, Bayard NU, Pan YG, Dwivedi G, Knox J, Glover RC, Rangel PC, Tanes C, Bittinger K, She Q, Hu H, Bonam SR, Maslanka JR, Planet PJ, Moustafa AM, Davis B, Chevrier A, Beattie M, Ni H, Blizard G, Furth EE, Mach RH, Lavertu M, Sellmyer MA, Tam Y, Abt MC, Weissman D, Zackular JP. Science 386(6717):69-75, Oct 2024. doi: 10.1126/science.adn4955. Epub 2024 Oct 3. PMID: 39361752; PMCID: PMC11719173. *first and co-corresponding author
Fast and facile synthesis of amidine-incorporated degradable lipids for versatile mRNA delivery in vivo
Han X*, Alameh MG*, Gong N, Xue L, Ghattas M, Bojja G, Xu J, Zhao G, Warzecha CC, Padilla MS, El-Mayta R, Dwivedi G, Xu Y, Vaughan AE, Wilson JM, Weissman D, Mitchell MJ. Nat Chem 16(10):1687-1697, Oct 2024. doi: 10.1038/s41557-024-01557-2. Epub 2024 Jul 9. PMID: 38982196; PMCID: PMC11446653. *equal contribution
Chitosan siRNA Nanoparticles Produce Significant Non-Toxic Functional Gene Silencing in Kidney Cortices
Alameh MG*, Tavakoli Naeini A, Dwivedi G, Lesage F, Buschmann MD, Lavertu M*. Polymers (Basel) 16(17):2547, Sep 2024. doi: 10.3390/polym16172547. PMID: 39274180; PMCID: PMC11398103. *co-corresponding authors
Enhancing in situ cancer vaccines using delivery technologies
Gong N, Alameh MG, El-Mayta R, Xue L, Weissman D, Mitchell MJ. Nat Rev Drug Discov 23(8):607-625, Aug 2024. doi: 10.1038/s41573-024-00974-9. Epub 2024 Jul 1. PMID: 38951662.
Rapid and definitive treatment of phenylketonuria in variant-humanized mice with corrective editing
Brooks DL, Carrasco MJ, Qu P, Peranteau WH, Ahrens-Nicklas RC, Musunuru K, Alameh MG*, Wang X*. Nat Commun 14(1):3451, Jun 2023. doi: 10.1038/s41467-023-39246-2. PMID: 37301931; PMCID: PMC10257655. *co-senior and co-corresponding authors
Lipid nanoparticles (LNP) induce activation and maturation of antigen presenting cells in young and aged individuals
Connors J, Joyner D, Mege NJ, Cusimano GM, Bell MR, Marcy J, Taramangalam B, Kim KM, Lin PJC, Tam YK, Weissman D, Kutzler MA, Alameh MG*, Haddad EK*. Commun Biol 6(1):188, Feb 2023. doi: 10.1038/s42003-023-04555-1. Erratum in: Commun Biol. 2025 Feb 22;8(1):285. doi: 10.1038/s42003-025-07603-0. PMID: 36805684; PMCID: PMC9936473. *co-senior and co-corresponding authors
Lipid nanoparticles enhance the efficacy of mRNA and protein subunit vaccines by inducing robust T follicular helper cell and humoral responses
Alameh MG*, Tombácz I*, Bettini E, Lederer K, Sittplangkoon C, Wilmore JR, Gaudette BT, Soliman OY, Pine M, Hicks P, Manzoni TB, Knox JJ, Johnson JL, Laczkó D, Muramatsu H, Davis B, Meng W, Rosenfeld AM, Strohmeier S, Lin PJC, Mui BL, Tam YK, Karikó K, Jacquet A, Krammer F, Bates P, Cancro MP, Weissman D, Luning Prak ET, Allman D, Locci M, Pardi N. Immunity 54(12):2877-2892, Dec 2021.e7. doi: 10.1016/j.immuni.2021.11.001. Epub 2021 Nov 4. Erratum in: Immunity. 2022 Jun 14;55(6):1136-1138. doi: 10.1016/j.immuni.2022.05.007. PMID: 34852217; PMCID: PMC8566475. *equal contribution
