PEOPLE

Jonni S. Moore, PhD

Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
University of Pennsylvania Perelman School of Medicine
Consultant, Flow Cytometry

Contact Information211 and 297 John Morgan Building
3620 Hamilton Walk
Philadelphia, PA, 19104
Office: (215) 898-6853
Fax: (215) 898-4227

Email: MOOREJ@MAIL.MED.UPENN.EDU

Specialty Division

Laboratory Medicine, Immunobiology and Experimental Pathology

Research Expertise

Research Summary

Research in our laboratory is focused on the contribution of failure of normal growth regulatory controls to the development of hematologic malignancies. Alterations in the balance of apoptosis and proliferation may lead to chronic leukemic states such as B cell chronic lymphocytic leukemia (B-CLL). Consistent genetic defects have been difficult to identify in B-CLL; hence our focus has been on alterations in cytokine networks which may affect the cellular environment of the malignant clone. We showed that TGFbeta, a potent immunosuppressive cytokine which can induce apoptosis and inhibit proliferation, is overproduced in mice and humans with B- CLL, but the leukemic B cells fail to respond to the apoptotic signals provided by TGFbeta. This defect in TGFbeta responsiveness is not due to a lack of expression of TGF receptors, but appears to be a failure in the signal transduction pathway for this cytokine. In addition to TGFbeta, we have investigated the role of T cell produced cytokines (IL4 and IFNg) in regulating the apoptotic process in CLL B cells.

We found a predominance of IFNg producing T cells in these patients as compared to normals, and that the leukemic B cells express higher levels of IFNg receptors. Interestingly, this cytokine has an extremely anti-apoptotic effect on the B cells. Thus, the leukemic B cell is producing a cytokine that can cause potent immunosuppression (and apoptosis) of other cells while being itself resistant and appears to be overly responsive to the anti-apoptotic effects of IFNg. Taken together, these aberrations could contribute to the survival and expansion of the malignant B cell. Current investigations are focused on the role of other cytokines in the control of hematologic malignancies, using both human and murine models, with the intent of identifying potent targets for therapy.

The laboratory is also involved in the development of new flow cytometric applications for research and clinical use. This includes basic and translational studies on applications for monitoring receptor/ligand binding, multiparameter functional studies, minimal residual disease detection, stem cell isolation and identification, and transplantation monitoring.

Current collaborators:
Peter Nowell, M.D. Pathology
Bruce Rosengard, M.D , Surgery
Alain Rook, M.D., Dermatology

Flow Cytometry Expertise

As Director of the Flow Cytometry and Cell Sorting Shared Resource and the Clinical Flow Cytometry Laboratory as well as in my own research, I have developed and applied a variety of sophisticated flow cytometric applications. This includes expertise in intracellular protein detection, complex data mining, cell proliferation, rare stem cell detection as well as other functional assays.

Graduate Groups

Immunology

Education

B.A. (Biology), University of Virginia, Charlottesville, 1972
Ph.D. (Microbiology), Thomas Jefferson University, Philadelphia, 1984

Specialty Certification

Becton-Dickinson FACScan Key Operator, 1990
Becton-Dickinson FACStar PLUS Key Operator, 1992

Postgraduate Training

Postdoctoral Fellow, Immunology Graduate Group, Dr. Peter C. Nowell and Dr. Richard G. Hoover, University of Pennsylvania School of Medicine, 1984-1988

Awards and Honors

Keynote Speaker Award, National Flow Cytometry Annual Course, Bowdoin College, Maine, 212
Sigma Xi Research Society (Associate Member), 1981
Sigma Xi Student Research Award, 1983
Thomas Jefferson University Fellow, 1983-1984
National Institutes of Health Postdoctoral Fellow, 1984-1987
Chapter Scholar, Arthritis Foundation, 1987, 1988, 1990, 1987-1990
Academy of Clinical Laboratory Physicians and Scientists, 2000-present
Peter C. Nowell Teaching Award, 2002
FOCUS Award for the Advancement of Women in Medicine, 2008
College of American Pathologists Outstanding Inspector, 2008
David B.P. Goodman, M.D., Ph.D. Award
Department of Pathology and Laboratory Medicine, 2009
Cyto2011 Pre-Congress Scientific Mentor, International Society for the Advancement of Cytometry, 2011
The Carleton and Sigrid Stewart Lectureship, Great Lakes International Imaging and Fluorescence Association, 2012
Wallace H. Coulter Award for Lifetime Achievement for Contributions to the Science, Education and Practice of Clinical Cytometry
International Clinical Cytometry Society, 2016

Memberships and Professional Organizations

Penn Professional Women, 1991 - Present
International Clinical Cytometry Society, 1996 - Present
Great Lakes International Imaging and Flow Cytometry Association, 1996 - Present
National Institutes of Health Special Emphasis Panel (SBIR), Hematology 1,2 Study Section, 1998 - 2005
University of Rochester School of Medicine, 2003 - 2003
Center for Human Cell Therapy, CBR Institute for Biomedical Research, Harvard University Medical School, 2004 - 2007
University of California at San Diego, Department of Pathology and Cancer Center, 2004 - 2004
College of Graduate Studies, Thomas Jefferson University, 2005 - Present
University of Alabama at Birmingham, Arthritis and Musculoskelatal Disease Center, 2005 - 2005
University of Michigan, Department of Pathology, 2006 - 2006
American Society for Investigative Pathology, 2006 - Present
American Association of Immunologists, - Present
International Society for Analytical Cytology, - Present
American College of Laboratory Physicians and Scientists, - Present
Association of Biomolecular Resource Facilities, - Present
International Society for Hematopathology and Graft Engineering, - Present

Web Links


Selected Publications

Extracellular Vesicles: Great Potential, Many Challenges

Nolan, JP and Moore, J, Cytometry Part B 90B(): 324-325, 2016

Vascular Health Profile Predicts Cardiovascular Outcomes in Patients with Diabetes

WT Rogers, L Zhang, S Welden, B Krieger, M Rickels, JS Moore, ER Mohler, III, Cytometry Part B, 2015

Endothelial Cell-, Platelet-, and Monocyte/Macrophage-Derived Microparticles are Elevated in Psoriasis Beyond Cardiometabolic Risk Factors.

Takeshita J, Mohler E, Krishnamoorthy P, Moore J, Rogers W, Zhang L, Gelfand J, Mehta N., J Am Heart Association, 2014

Personalized cytomic assessment of vascular health: Evaluation of the vascular health profile in diabetes mellitus.

Kurtzman N, Zhang L, French B, Jonas R, Bantly A, Rogers WT, Moore JS, Rickels M, Mohler ER III, Cytometry Part B: Clinical Cytometry in press(): , 2013

Potent Obatoclax cytotoxicity and activation of triple death mode killing across infant acute lymphoblastic leukemia. Blood 121(14): 2689-2703

Urtishak KA, Edwards AY, Wang LS, Hudome A, Robinson BW, Barrett JS, Cao K, Cory L, Moore JS, Bantly AD, Yu QC, Chen IM, Atlas SR, Willman CL, Kundu M, Carroll AJ, Heerema NA, Devidas M, Hilden JM, Dreyer ZE, Hunger SP, Reaman GH, Felix CA, Blood 121(14): 2689-2703, 2013

Endothelial Microparticles; Sophisticated Vesicles Modulating Vascular Function

Curtis A.M., Edelberg J., Jonas R., Rogers W.T., Moore J.S., Wajihuddin S., Mohler E.R. III, Vascular Medicine in press(): , 2013

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Tario Jr., J. D., Humphrey, K., Bantly, A. D., Muirhead, K. A., Moore, J. S., Wallace, P. K, J. Vis. Exp. 70(): e4287, 2012

Elevated endothelial cell, platelet and T-cell micro particles in psoriasis may provide novel link to atherosclerosis free

Mehta NN, Li RC, Takeshita J, Zhang L, VanVoorhees A, Rogers W, Wilcox M, Raper A, Moore J, Gelfand JM, Mohler ER, Journal of the American College of Cardiology 59(13sI): E2055, 2012

Effect of darbepoetin alfa on endothelial progenitor cells and vascular reactivity in chronic kidney disease.

Mohler ER III, Zhang L, Medenilla E, Rogers W, French B, Bantly A, Moore JS, Huan Y, Murashima M, Berns JS, Vascular Medicine 16(13): 183-189, 2011

Circulating endothelial progenitor cells are reduced in SLE in the absence of coronary artery calcification.

Baker JF, Zhang L, Imadojemu S, Sharpe A, Patil S, Moore JS, Mohler ER 3rd, Von Feldt J., Rheumatol Int., 2011, PMID:21246370

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