Donald L. Siegel, PhD, MD

Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Director, Division of Transfusion Medicine and Therapeutic Pathology
Director, Fellowship Program in Transfusion Medicine
Director, Clinical Cell and Vaccine Production Facility (CVPF)
Center for Advanced Cellular Therapeutics (CACT)
University of Pennsylvania Perelman School of Medicine

Contact Information9-112 South Tower, Big 421
3400 Civic Center Blvd
Philadelphia, PA, 19104
Office: (215) 662-3942


Specialty Division

Transfusion Medicine & Therapeutic Pathology, Immunobiology and Experimental Pathology

Research Expertise

Research Interests

Discovery and characterization of diagnostic and therapeutic monoclonal antibodies

Research Summary

My laboratory studies human immune repertoires in health and disease using phage display technologies for the production of human and non-human monoclonal antibodies relevant to transfusion medicine, benign hematology, infectious diseases, and oncology. Current studies focus on developing targeted drug delivery methods using red cell antibodies coupled to novel anti-thrombotic/anti-inflammatory molecules; cloning monoclonal primate neutralizing Dengue, Ebola, MERS and Zika virus antibodies for human in vivo antibody gene expression therapy; and isolating recombinant human and humanized antibody fragments for the design of T-cell chimeric antigen receptors for cancer therapy.

Clinical Expertise

transfusion medicine, blood banking, apheresis, hematopoietic stem cell collection/processing, cGMP manufacture of cellular therapies for phase I first-in-human clinical trials

Itmat Expertise

Dr. Siegel's research focuses on the study of the molecular genetics of human auto- and alloantibodies responsible for blood cell-related disorders such as immune cytopenias (ITP, AIHA, TTP), hemolytic transfusion reactions, and hemolytic disease of the newborn. The Siegel laboratory has developed several phage display-related technologies for the cloning of human immune repertoires and the production of human monoclonal antibodies. These studies have led to the development of novel diagnostic reagents and antibodies with therapeutic potential.


Sc.B. (Biophysics), Brown University, 1977
Ph.D. (Biophysics), Harvard University, 1983
M.D. University of Pennsylvania, 1987

Specialty Certification

The American Board of Pathology, 1992

Postgraduate Training

Resident in Clinical Pathology, HUP, Philadelphia, PA, 1987-1989
Chief Resident, Division of Laboratory Medicine, HUP, Philadelphia, PA, 1989-1990
Fellowship, Transfusion Medicine, HUP, Philadelphia, PA, 1990-1991
Academic Medical Leadership Program, The Wharton School of the University of Pennsylvania, Philadelphia, PA, 2007-2008
Penn Medicine Leadership Forum, The Wharton School of the University of Pennsylvania, Philadelphia, PA, 2008-2009

Awards and Honors

Sigma Xi, 1977
Honors in Biophysics, Brown University, undergraduate thesis: "Application of non-equilibrium thermodynamics to plasma membrane transport", 1977
NIH Pre-Doctoral Fellowship in the laboratory of Dr. Daniel Branton, Harvard University, Ph.D. dissertation: "Purification and characterization of human erythrocyte band 4.9, an actin bundling protein", 1977-1983
Young Investigator Award, First Prize Recipient, Boston Blood Club, Harvard Medical School, 1983
Measey Foundation Scholar, University of Pennsylvania School of Medicine, 1983-1985
2 Young Investigator Awards, Academy of Clinical Laboratory Physicians and Scientists, 1988 Annual Meeting, 1988
Berwick Resident Teaching Award, Department of Pathology & Laboratory Medicine, University of Pennsylvania, 1990
Julius Erving Research Award of the Lupus Foundation of the Delaware Valley, 1991-1992
Clinical Investigator Award (K08), National Heart, Lung, and Blood Institute, NIH, 1991-1994
National Blood Foundation Research Award, 1991-1992
Basil O'Connor Scholar Research Award, March of Dimes Birth Defects Foundation, 1993-1995
National Blood Foundation Scholar, 1996
National Blood Foundation Research Award, 1996-1997
Peter C. Nowell Teaching Award, Department of Pathology & Laboratory Medicine, University of Pennsylvania, 1998
Leonard Berwick Memorial Teaching Award, University of Pennsylvania School of Medicine, 2000
Elected to John Morgan Society, 2001
Philadelphia Antiques Show Proceeds Awardee, Board of Women’s Visitors, University of Pennsylvania Medical Center, 2003
Pennsylvania Biotechnology Greenhouse Award (translational research), 2003-2005
Christian R. and Mary F. Lindback Foundation Award for Distinguished Teaching, University of Pennsylvania, 2003
Patient Health Care Hero, Penn Medicine, 2010
Elected to The Academy of Master Clinicians, Penn Medicine, 2013
Patient Health Care Hero, Penn Medicine, 2015
UPenn Health System Quality & Patient Safety Award, “Blood Delivery and Wastage in the PeriOperative Setting”, 2016
RISE (Research Innovation in Scientific Excellence) Award from AABB for best original research article published in Transfusion in 2016, 2017
Gift of Life Award, Lee Wynn Foundation, 2018
Francis S. Morrison, MD Memorial Lectureship, American Association for Apheresis, 2018
Honorary Lecturer Award, American Association of Apheresis, 2020
Robert de Villiers Spiral of Life Award, Leukemia & Lymphoma Society, 2020
Patient Health Care Hero, Penn Medicine, 2020
Dale E. Smith Memorial Award, National Blood Foundation, American Association of Blood Banks, 2020
Tibor Greenwalt Memorial Award and Lectureship, American Association of Blood Banks, 2021

Memberships and Professional Organizations

American Association for Cell Biology, 1981 - 2008
American Society of Hematology, 1987 - present
American Association of Blood Banks, 1989 - present
Scientific Review Panel, Program Project on Prion Diseases, National Institute on Aging, 1998 - 1998
NIH/AABB-Sponsored Scientific Think Tank on "Setting the Research Agenda for Immunobiology in Transfusion Medicine", 1999 - 2000
Scientific Subsection Committee, American Association of Blood Banks, 1999 - present
"Structural Analysis of Monoclonal Antibodies to Blood Group Antigens" for 4th International Workshop on Monoclonal Antibodies Against Human Red Blood Cells and Related Antigens, Paris, 1999 - 2001
Scientific Subcommittee on Hemoglobin/Red Cell, American Society of Hematology, 1999 - 2003
Medical Advisory Committee, Penn-Jersey Division of the American Red Cross, 2000 - present
John Morgan Society, 2001 - present
American Society for Apheresis, 2001 - present
PhenoTech, Inc. (UPenn start-up), 2003 - 2009
Clinical Applications and Standards Committee, American Society for Apheresis, 2004 - present
Hema-Quebec, Quebec City, Canada, 2004 - 2005
NIH NHLBI Strategic Planning Meeting "Research in Global Blood Safety and Availability, Domestic and International", 2006 - 2007
FDA Workshop on Molecular Methods in Transfusion Medicine, 2006 - 2007
NIH Hematology Small Business Activities [SBIR/STTR] Special Emphasis Panel, 2006 - 2010
Hema-Quebec, Quebec City, Canada, 2006 - 2007
Executive Committee of the Medical Advisory Board, Penn-Jersey Division of the American Red Cross, 2008 - present
NIH, Member, Selected Topics in Transfusion Medicine Study Section, 2010 - Present
NIH Study Section "Selected Topics in Transfusion Medicine" (ZRG1 VH-E 50R), 2010 - Present
International Society for Cellular Therapy, 2017 - present
Department of Transfusion Medicine, National Institutes of Health, 2017 - Present
NIH NHLBI NITM NRSA Institution Research Training Grant (T32), 2019 - Present
NIH NHLBI NITM NRSA Institution Research Training Grant (T32), 2020 - 2024

Web Links

Selected Publications

Transfusion Medicine Meets Cell & Gene Therapy: ISCT Featured Member

Don Siegel, ISCT Telegraft, 2020

Your Guide to Donating Blood During Coronavirus – And After

Don Siegel, Shape Magazine, 2020

Controversial Uses of Blood Components

Don Siegel, AABB, 2020

How One NBF Grant Recipient Proliferated His Success in Many Different Aspects of His Career

Don Siegel, AABB News, 2020

Linking CART19 immunotherapy outcome with genomic modification by vector integration

Nobles, C., Sherrill-Mix, S., Everett, J.K., Reddy, S., Fraietta, J., Porter, D., Frey, N., Gill, S.I., Grupp, S., Maude, S., Siegel, D.L., Levine, B.L. June, C.H., Lacey, S., Melenhorst, J., Bushman, F., J Clin Invest 130(): 673-685, 2020

Characterization of autologous T cells engineered to express NY-ESO-1 TCR with multiplexed CRISPR/Cas9 editing (NYCE T cells

Xu, J., Gohil, M., Stadtmauer, E., Fraietta, J.A., Gonzalez, V.E., Salas-McKee, J., Jadlowsky, J., Gladney. W., Lamontagne, A., Fesnak, A., Siegel, D. Levine. B.L., Lacey, S., June, C., Davis, M.M., Cytotherapy 22(): S35-S36, 2020

Diagnostic biomarkers to differentiate sepsis from cytokine release syndrome in critically ill children

Diorio, C., Shaw, P., Pequignot, E., Orlenko, A., Chen, F., Aplenc, R., Barrett, D.M., Bassiri, H., Edward Behrens, E., Gonzalez, V., Koterba, N., Levine, B.L., Maude, S.L., Meyer, N.J., Moore, J.H., Paessler, M., Porter, D.L., Rush, J., Siegel, D.L., Suhoski, M., Zhang, D., June, C.H., Grupp, S.A., Melenhorst, J.J., Lacey, S.F., Weiss, S.L., Teachey, D.T., Blood Adv in press(): , 2020

Bi-specific and split CAR T cells targeting CD13 and TIM3 eradicate acute myeloid leukemia

61. He, X., Feng, Z., Ma, J., Ling, S., Cao, Y., Gurung, B., Wu, Y., Katona, B.W., O’Dwyer, K., Siegel, D.L., June, C.H., Hua, X., Blood 135(): 713-723, 2020

CRISPR-engineered T cells in patients with refractory cancer

Stadtmauer, E.A., Fraietta, J.A., Davis, M.M., Cohen, A.D., Weber, K.L., Lancaster, E., Mangan, P.A., Kulikovskaya, I., Gupta, M., Chen, F., Tian, L., Gonzalez, V.E., Xu, J., Jung, I.Y., Melenhorst, J.J., Plesa, G., Shea, J., Matlawski, T., Cervini, A., Gaymon, A.L., Desjardins, S., Lamontagne, A., Salas-Mckee, J., Fesnak, A., Siegel, D.L., Levine, B.L., Jadlowsky, J.K., Young, R.M., Chew, A., Hwang, W.-T., Hexner, E.O., Carreno, B.M., Nobles, C.L., Bushman, F.D., Parker, K.R., Qi, Y., Satpathy, A.T., Chang, H.Y., Zhao, Y., Lacey, S.F., June, C.H, Science 367(): eaba7365, 2020

Synthetic nucleic acid antibody prophylaxis confers rapid and durable protective immunity against Zika virus challenge

Choi, H., Kudchodkar, S.B., Reuschel, E.L., Asija, K., Borole, P., Agarwal, S., Van Gorder, L., Reed, C.C., Gulendram, G., Ramos, S., Broderick, K.E., Kim, J.J., Ugen, K.E., Kobinger, G., Siegel, D.L., Weiner, D.B., Muthumani, K., Human Vaccines & Immunotherapeutics 16(): 907-918, 2020

Search PubMed for articles