Michael C. Milone, MD, PhD

Associate Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
Associate Director, Toxicology Laboratory
University of Pennsylvania Perelman School of Medicine

Contact Information3400 Spruce Street 7103 Founders Pavilion
Philadelphia, PA 19104
Office: 215-662-6575
Fax: 215-662-7529


Specialty Division

Laboratory Medicine, Immunobiology and Experimental Pathology

Research Expertise

Research in my laboratory is focused on T cell biology and applications of genetically-engineered T cells to the treatment of disease. Active research projects in my laboratory include:

1) Developing chimeric antigen receptors (CARs) for adoptive immunotherapy of cancer with enhanced function and improved safety.

2) Developing and applying synthetic immunoreceptors to the treatment of immune-mediated disease.

3) Exploring the role of co-stimulatory signals in directing T cell metabolism and the way this metabolism affects T cell function within tumors.


Clinical Expertise

Clinical Laboratory Toxicology, Therapeutic Drug Monitoring, Clinical Chemistry, Blood Banking/Transfusion Medicine, Therapeutic Apheresis

Itmat Expertise

My laboratory is focused upon developing methods to use the immune system to treat cancer. Active research includes 1) exploring the mechanical properties of artificial adhesion substrates used in T cell culture and their influence on T cell biology, 2) development of artificial antigen receptors and signaling systems for control of T cell specificity

Graduate Groups

Cell and Molecular Biology


B.S (Chemical Biology), Stevens Institute of Technology, 1993
M.D. (Medical Doctor), UMDNJ- New Jersey Medical School, 1999
Ph.D (Experimental Pathology/ Immunology), UMDNJ - Graduate School of Biomedical Sciences, 1999

Specialty Certification

Clinical Pathology, American Board of Pathology, 2002

Postgraduate Training

Internship, Internal Medicine, Hospital of the University of Pennsylvania, 1999-2000
Resident, Clinical Pathology, Hospital of the University of Pennsylvania, 2000-2002
Chief Resident, Division of Laboratory Medicine, Hospital of the University of Pennsylvania, 2001-2002
Clinical Fellowship, Blood Banking and Transfusion Medicine, Hospital of the University of Pennsylvania, 2002-2003
Postdoctoral. Research Fellow, Translational Research Program, Abramson Family Cancer Institute, University of Pennsylvania School of Medicine, 2003-2007
Clinical Fellow, Clinical Chemistry and Laboratory Toxicology, Hospital of the University of Pennsylvania, 2004-2007

Awards and Honors

Stanley S. Bergen Award for Academic Excellence, UMDNJ, 1996
Faculty Organization Award, UMDNJ-New Jersey Medical School, 1999
Stanely S. Bergen Medal of Excellence, UMDNJ, 1999
The Peter C. Nowell Teaching Award, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 2011
Top Ten Clinical Research Achievement Award, Clinical Research Forum, 2017
Top 20 Clinical Research Papers for 2019, Clinical Research Forum, 2019
Though Leader Award, Agilent Technologies, 2020

Memberships and Professional Organizations

American Association of Clinical Chemistry, 2007 - Present
American Society of Clinical Pharmacology and Therapeutics, 2007 - 2010
Academy of Clinical Laboratory Physicians and Scientists, 2008 - 2015
College of American Pathologists, 2008 - Present
American Society of Hematology, 2008 - Present
American Association of Immunologists, 2009 - Present
International Association of Therapeutic Drug Monitoring and Clinical Toxicology, 2009 - Present
American Society of Gene and Cell Therapy, 2009 - Present
American Society of Clinical Pathology, 2015 - Present
National Institute for innovation in Manufacturing Biopharmaceuticals (NIIMBL), 2017 - Present

Web Links

Selected Publications

Changes in Bone Marrow Tumor and Immune Cells Correlate with Durability of Remissions Following BCMA CAR T Therapy in Myeloma.

Dhodapkar KM, Cohen AD, Kaushal A, Garfall AL, Manalo RJ, Carr AR, McCachren SS, Stadtmauer EA, Lacey SF, Melenhorst JJ, June CH, Milone MC, Dhodapkar MV., Blood Cancer Discov., 2022

Therapeutic Drug Monitoring

Michael C. Milone and Leslie Shaw, Teitz Textbook of Clinical Chemistry and Molecular Diagnostics, 7th ed., 2022

An Epidemic within a Pandemic: An 8-Year-Old Boy with Xylazine-Complicating Fentanyl Poisoning, and Drug Detection Challenges

Gill, E. L., Mack, E. A., Osterhoudt, K. C., Shaw, L. M., Milone, M. C., J Appl Lab Med, 2022

Rapid manufacturing of non-activated potent CAR T cells

Ghassemi, Saba, Durgin, Joseph S., Nunez-Cruz, Selene, Patel, Jai, Leferovich, John, Pinzone, Marilia, Shen, Feng, Cummins, Katherine D., Plesa, Gabriela, Cantu, Vito Adrian, Reddy, Shantan, Bushman, Frederic D., Gill, Saar I., O’Doherty, Una, O’Connor, Roddy S., Milone, Michael C., Nature Biomedical Engineering, 2022

CRISPR/Cas9-mediated Gene Knockout Followed by Negative Selection Leads to a Complete TCR Depletion in ortho CAR19 T Cells

Zhang, Q., Yang, J., Manoharan, Enea, Yu, A. B., Milone, M. C., Bio Protoc 12(15): , 2022

Targeting PARP11 to avert immunosuppression and improve CAR T therapy in solid tumors

Zhang, H., Yu, P., Tomar, V. S., Chen, X., Atherton, M. J., Lu, Z., Zhang, H. G., Li, S., Ortiz, A., Gui, J., Leu, N. A., Yan, F., Blanco, A., Meyer-Ficca, M. L., Meyer, R. G., Beiting, D. P., Li, J., Nunez-Cruz, S., O'Connor, R. S., Johnson, L. R., Minn, A. J., George, S. S., Koumenis, C., Diehl, J. A., Milone, M. C., Zheng, H., Fuchs, S. Y., Nat Cancer 3(7): 808-820, 2022

The IAP antagonist birinapant enhances chimeric antigen receptor T cell therapy for glioblastoma by overcoming antigen heterogeneity

Song, E. Z., Wang, X., Philipson, B. I., Zhang, Q., Thokala, R., Zhang, L., Assenmacher, C. A., Binder, Z. A., Ming, G. L., O'Rourke, D. M., Song, H., Milone, M. C., Mol Ther Oncolytics 27(): 288-304, 2022

Anti-BCMA/CD19 CAR T cells with early immunomodulatory maintenance for multiple myeloma responding to initial or later-line therapy

Garfall, A. L., Cohen, A. D., Susanibar-Adaniya, S. P., Hwang, W. T., Vogl, D. T., Waxman, A. J., Lacey, S. F., Gonzalez, V. E., Fraietta, J. A., Gupta, M., Kulikovskaya, I., Tian, L., Chen, F., Koterba, N., Bartoszek, R. L., Patchin, M., Xu, R., Plesa, G., Siegel, D. L., Brennan, A., Nelson, A. M., Ferthio, R., Cosey, A., Shea, K. M., Leskowitz, R., Four, M., Wilson, W. V., Miao, F., Lancaster, E., Carreno, B. M., Linette, G. P., Hexner, E. O., Young, R. M., Bu, D., Mansfield, K. G., Brogdon, J. L., June, C. H., Milone, M. C., Stadtmauer, E. A., Blood Cancer Discov, 2022

Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation

Ellis, G. I., Deng, M. Z., Winn, D. W., Coker, K. E., Shukla, D., Bhoj, V., Milone, M. C., Duran-Struuck, R., Riley, J. L., STAR Protoc 3(4): 101784, 2022

Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque

Ellis, G. I., Coker, K. E., Winn, D. W., Deng, M. Z., Shukla, D., Bhoj, V., Milone, M. C., Wang, W., Liu, C., Naji, A., Duran-Struuck, R., Riley, J. L., Cell Rep Med 3(5): 100614, 2022