PEOPLE

Michael C. Milone, MD, PhD

Associate Professor of Pathology and Laboratory Medicine at the
Hospital of the University of Pennsylvania
Associate Director, Toxicology Laboratory
Perelman School of Medicine at the University of Pennsylvania 

Associate Vice Chair for Faculty Development & Academic Affairs

Contact InformationHospital of the University of Pennsylvania
Department of Pathology and Laboratory Medicine
7.103 Founders Pavilion
3400 Spruce Street
Philadelphia, PA 19104
Tel: 215-662-6575
Fax: 215-662-7529
Email: milone@pennmedicine.upenn.edu

Specialty Division

Laboratory Medicine, Cancer and Immunobiology

Research Expertise

Research in my laboratory is focused on T cell biology and applications of genetically-engineered T cells to the treatment of disease. Active research projects in my laboratory include:

1) Developing chimeric antigen receptors (CARs) for adoptive immunotherapy of cancer with enhanced function and improved safety.

2) Developing and applying synthetic immunoreceptors to the treatment of immune-mediated disease.

3) Exploring the role of co-stimulatory signals in directing T cell metabolism and the way this metabolism affects T cell function within tumors.

 

Clinical Expertise

Clinical Laboratory Toxicology, Therapeutic Drug Monitoring, Clinical Chemistry, Blood Banking/Transfusion Medicine, Therapeutic Apheresis

Itmat Expertise

My laboratory is focused upon developing methods to use the immune system to treat cancer. Active research includes 1) exploring the mechanical properties of artificial adhesion substrates used in T cell culture and their influence on T cell biology, 2) development of artificial antigen receptors and signaling systems for control of T cell specificity

Graduate Groups

Cell and Molecular Biology

Education

B.S (Chemical Biology), Stevens Institute of Technology, 1993
M.D. (Medical Doctor), UMDNJ- New Jersey Medical School, 1999
Ph.D (Experimental Pathology/ Immunology), UMDNJ - Graduate School of Biomedical Sciences, 1999

Specialty Certification

Clinical Pathology, American Board of Pathology, 2002

Postgraduate Training

Internship, Internal Medicine, Hospital of the University of Pennsylvania, 1999-2000
Resident, Clinical Pathology, Hospital of the University of Pennsylvania, 2000-2002
Chief Resident, Division of Laboratory Medicine, Hospital of the University of Pennsylvania, 2001-2002
Clinical Fellowship, Blood Banking and Transfusion Medicine, Hospital of the University of Pennsylvania, 2002-2003
Postdoctoral. Research Fellow, Translational Research Program, Abramson Family Cancer Institute, University of Pennsylvania School of Medicine, 2003-2007
Clinical Fellow, Clinical Chemistry and Laboratory Toxicology, Hospital of the University of Pennsylvania, 2004-2007

Awards and Honors

Stanley S. Bergen Award for Academic Excellence, UMDNJ, 1996
Stanely S. Bergen Medal of Excellence, UMDNJ, 1999
Faculty Organization Award, UMDNJ-New Jersey Medical School, 1999
The Peter C. Nowell Teaching Award, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 2011
Top Ten Clinical Research Achievement Award, Clinical Research Forum, 2017
Top 20 Clinical Research Papers for 2019, Clinical Research Forum, 2019
Though Leader Award, Agilent Technologies, 2020

Memberships and Professional Organizations

American Society of Clinical Pharmacology and Therapeutics, 2007 - 2010
American Association of Clinical Chemistry, 2007 - Present
Academy of Clinical Laboratory Physicians and Scientists, 2008 - 2015
College of American Pathologists, 2008 - Present
American Society of Hematology, 2008 - Present
American Society of Gene and Cell Therapy, 2009 - Present
International Association of Therapeutic Drug Monitoring and Clinical Toxicology, 2009 - Present
American Association of Immunologists, 2009 - Present
American Society of Clinical Pathology, 2015 - Present
National Institute for innovation in Manufacturing Biopharmaceuticals (NIIMBL), 2017 - Present

Web Links


Selected Publications

Bendamustine Lymphodepletion Before Axicabtagene Ciloleucel Is Safe and Associates with Reduced Inflammatory Cytokines

Ghilardi G, Paruzzo L, Svoboda J, Chong EA, Shestov AA, Chen L, Cohen IJ, Gabrielli G, Nasta SD, Porazzi P, Landsburg DJ, Gerson JN, Carter J, Barta SK, Yelton RD, Pajarillo R, Patel V, White G, Ballard H, Weber E, Napier EB, Chong ER, Fraietta JA, Garfall A, Porter DL, Milone MC, O'Connor RS, Schuster SJ, Ruella M., Blood Adv, 2023, PMID:38113468

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Immunotherapy targeting B cells and long-lived plasma cells effectively eliminates pre-existing donor-specific allo-antibodies

Zhang Z, Markmann C, Yu M, Agarwal D, Rostami S, Wang W, Liu C, Zhao H, Ochoa T, Parvathaneni K, Xu X, Li E, Gonzalez V, Khadka R, Hoffmann J, Knox JJ, Scholler J, Marcellus B, Allman D, Fraietta JA, Samelson-Jones B, Milone MC, Monos D, Garfall AL, Naji A, Bhoj VG., Cell Rep Med 4(): 101336, 2023, PMID:38118406

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F77 antigen is a promising target for adoptive T cell therapy of prostate cancer

Grover P, Nunez-Cruz S, Leferovich J, Wentz T, Bagchi A, Milone MC, Greene MI., Biochem Biophys Res Commun 680(): 51-60, 2023, PMID:37717341

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Association of Urine Fentanyl Concentration With Severity of Opioid Withdrawal Among Patients Presenting to the Emergency Department

Thakrar AP, Faude S, Perrone J, Milone MC, Lowenstein M, Snider CK, Spadaro A, Delgado MK, Nelson LS, Kilaru AS., J Addict Med, 2023, PMID:36880886

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Anti-BCMA/CD19 CAR T Cells with Early Immunomodulatory Maintenance for Multiple Myeloma Responding to Initial or Later-Line Therapy

Garfall AL, Cohen AD, Susanibar-Adaniya SP, Hwang WT, Vogl DT, Waxman AJ, Lacey SF, Gonzalez VE, Fraietta JA, Gupta M, Kulikovskaya I, Tian L, Chen F, Koterba N, Bartoszek RL, Patchin M, Xu R, Plesa G, Siegel DL, Brennan A, Nelson AM, Ferthio R, Cosey A, Shea KM, Leskowitz R, Four M, Wilson WV, Miao F, Lancaster E, Carreno BM, Linette GP, Hexner EO, Young RM, Bu D, Mansfield KG, Brogdon JL, June CH, Milone MC, Stadtmauer EA., Blood Cancer Discov 4(): 118-133, 2023, PMID:36413381

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Precision targeting of autoantigen-specific B cells in muscle-specific tyrosine kinase myasthenia gravis with chimeric autoantibody receptor T cells

Oh S, Mao X, Manfredo-Vieira S, Lee J, Patel D, Choi EJ, Alvarado A, Cottman-Thomas E, Maseda D, Tsao PY, Ellebrecht CT, Khella SL, Richman DP, O'Connor KC, Herzberg U, Binder GK, Milone MC, Basu S, Payne AS., Nat Biotechnol, 2023, PMID:36658341

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CD38 as a pan-hematologic target for chimeric antigen receptor T cells

Glisovic-Aplenc, T., Diorio, C., Chukinas, J. A., Veliz, K., Shestova, O., Shen, F., Nunez-Cruz, S., Vincent, T. L., Miao, F., Milone, M. C., June, C. H., Teachey, D. T., Tasian, S. K., Aplenc, R., Gill, S., Blood Adv 7(16): 4418-4430, 2023

Changes in Bone Marrow Tumor and Immune Cells Correlate with Durability of Remissions Following BCMA CAR T Therapy in Myeloma.

Dhodapkar KM, Cohen AD, Kaushal A, Garfall AL, Manalo RJ, Carr AR, McCachren SS, Stadtmauer EA, Lacey SF, Melenhorst JJ, June CH, Milone MC, Dhodapkar MV., Blood Cancer Discov., 2022

Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque

Ellis, G. I., Coker, K. E., Winn, D. W., Deng, M. Z., Shukla, D., Bhoj, V., Milone, M. C., Wang, W., Liu, C., Naji, A., Duran-Struuck, R., Riley, J. L., Cell Rep Med 3(5): 100614, 2022

Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation

Ellis, G. I., Deng, M. Z., Winn, D. W., Coker, K. E., Shukla, D., Bhoj, V., Milone, M. C., Duran-Struuck, R., Riley, J. L., STAR Protoc 3(4): 101784, 2022