Flow Cytometry

The Flow Cytometry Laboratory performs rapid identification and quantification of subsets of cells using laser-activated fluoresecent antibodies to cell surface markers. Using up to eight different markers simultaneously, abnormal populations of cells can be identified with a high level of sensitivity to aid in the diagnosis of leukemias and lymphomas. Other tests offered include quantitation of CD4 T-cells for monitoring of HIV, measurement of hematopoietic stem cells, measurement of maternal-fetal bleed, and evaluation of cellular subsets for immunodeficiency diagnosis.

The Flow Cytometry Lab is also part of the Abramson Cancer Center and Cell Sorting Shared Resource as a core facility at the University of Pennsylvania. The lab is dedicated to providing all Penn investigators access to high-quality, cost-effective flow cytometric services, and the scientific expertise necessary to use this technology in their research effort. It is the largest academic flow cytometry laboratory in the country, housing four cell sorters, eight tabletop analyzers, and a staff of five support investigators.