Human Immunology

The Human Immunology Core provides expertise and instrumentation for the analysis of immune function in patients during treatment with anticancer drugs and immunotherapy. The Core serves as a central facility for incorporating the newest technologies and performing validated immunology assays for phase I/II clinical studies, while also offering expert scientific and technical consultations to users interested in conducting this kind of innovative research.

Current services include:

• Multiplex Bead Arrays (e.g., luminex) which allow the simultaneous characterization of multiple cytokines or other biomarkers
• Digital ELISA (Quanterix HDX) for high-sensitivity protein detection
• Serology assays by ELISA, electrochemiluminescence or cell-based assay (e.g., ANA)
• Immune repertoire profiling (BCR and TCR sequencing with bulk or single cell platforms)
• Flow-cytometry based immunophenotyping and proliferation (CFSE) assays
• Cellular assays such as B and T cell ELISpot
• Primary lymphocyte isolation and sub-population enrichment from HLA-typed leukapheresis donors
• Processing, viable cryopreservation and banking of cells (e.g., blood, PBMCs, tumor samples etc.) and fluids (serum, plasma, CSF etc.) for clinical trial mechanistic assays
• QC, processing, analysis and visualization of immunology data including immune repertoire, single cell RNA-seq,luminex and flow cytometry

Please contact the Core for consultations for projects, method selection, clinical trial sample collection, budget preparation and letters of collaboration for grant proposals when designing your project.

Please cite our RRID number when acknowledging the Core in your publications: RRID: SCR_022380.