Jonni S. Moore, PhD

Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
University of Pennsylvania Perelman School of Medicine
Consultant, Flow Cytometry

Contact Information211 and 297 John Morgan Building
3620 Hamilton Walk
Philadelphia, PA, 19104
Office: (215) 898-6853
Fax: (215) 898-4227


Specialty Division

Laboratory Medicine, Hematopathology, Immunobiology and Experimental Pathology

Research Expertise

Research Summary

Research in our laboratory centers on technology development with a focus on complex technologies, in particular cytomics. We pioneered new technology in flow cytometry liquid biopsies including the detection of extracellular vesicles as a rich source of prognostic and diagnostic information and developing this technology for diagnostics/prognostics in cardiovascular disease, traumatic brian injury and relapsing breast cancer. Most recently she has focused on the development of high dimensional deep phenotyping approaches for the identification of biomarkers of cancer responses using advance cytomic technology.
Current collaborators:
Lewis Chodosh, MD, PhD, Cancer Biology
Erica Carpenter, MBA, PhD, Department of Medicine
John Wherry, Parker Institute for Cancer Immunotherapy
Mona Kumar, MD, Neurology

Flow Cytometry Expertise

As Director of the Penn Cytomics and Cell Sorting Shared Resource and founder of the Hospital of the University of Pennsylvania Clinical Flow Cytometry Laboratory as well as in my own research, I have developed and applied a variety of sophisticated flow cytometric applications with focus on translational application and computational interpretation. This includes expertise in intracellular protein detection, complex data mining, cell proliferation, rare stem cell detection as well as other functional assays. My lab has been recognized as "exceptional" by the NCI for over 20 years.

Graduate Groups



B.A. (Biology), University of Virginia, Charlottesville, 1972
Ph.D. (Microbiology), Thomas Jefferson University, Philadelphia, 1984

Specialty Certification

Becton-Dickinson FACScan Key Operator, 1990
Becton-Dickinson FACStar PLUS Key Operator, 1992

Postgraduate Training

Postdoctoral Fellow, Immunology Graduate Group, Dr. Peter C. Nowell and Dr. Richard G. Hoover, University of Pennsylvania School of Medicine, 1984-1988

Awards and Honors

Keynote Speaker Award, National Flow Cytometry Annual Course, Bowdoin College, Maine, 212
Sigma Xi Research Society (Associate Member), 1981
Thomas Jefferson University Fellow, 1983-1984
Sigma Xi Student Research Award, 1983
National Institutes of Health Postdoctoral Fellow, 1984-1987
Chapter Scholar, Arthritis Foundation, 1987, 1988, 1990, 1987-1990
Academy of Clinical Laboratory Physicians and Scientists, 2000-present
Peter C. Nowell Teaching Award, 2002
College of American Pathologists Outstanding Inspector, 2008
FOCUS Award for the Advancement of Women in Medicine, 2008
David B.P. Goodman, M.D., Ph.D. Award
Department of Pathology and Laboratory Medicine, 2009
Cyto2011 Pre-Congress Scientific Mentor, International Society for the Advancement of Cytometry, 2011
The Carleton and Sigrid Stewart Lectureship, Great Lakes International Imaging and Fluorescence Association, 2012
Wallace H. Coulter Award for Lifetime Achievement for Contributions to the Science, Education and Practice of Clinical Cytometry
International Clinical Cytometry Society, 2016
ISAC 2018 Membership Award for Advancing the Field of Cytometry, International Society for the Advancement of Cytometry, 2018
Goodman Award for University Service, Department of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania, 2018
EXCEPTIONAL SHARED RESOURCE designation by NCI in Abramson Cancer Center comprehensive cancer center competitive renewal, 2020-2025

Memberships and Professional Organizations

American Association of Immunologists, 1985 - present
Penn Professional Women, 1991 - Present
International Society for Advancement of Cytometry, 1992 - Present
International Clinical Cytometry Society, 1996 - Present
Great Lakes International Imaging and Flow Cytometry Association, 1996 - Present
National Institutes of Health Special Emphasis Panel (SBIR), Hematology 1,2 Study Section, 1998 - 2005
International Society for Hematopathology and Graft Engineering, 1999 - 2019
University of Rochester School of Medicine, 2003 - 2003
Center for Human Cell Therapy, CBR Institute for Biomedical Research, Harvard University Medical School, 2004 - 2007
University of California at San Diego, Department of Pathology and Cancer Center, 2004 - 2004
College of Graduate Studies, Thomas Jefferson University, 2005 - 2019
University of Alabama at Birmingham, Arthritis and Musculoskelatal Disease Center, 2005 - 2005
American Society for Investigative Pathology, 2006 - present
University of Michigan, Department of Pathology, 2006 - 2006
New York University Grossman School of Medicine, Flow Cytometry and Cell Sorting Shared Resource, 2011 - present
Association of Biomolecular Resource Facilities, - Present

Web Links

Selected Publications

Implementation of a single cell double-emulsion droplet assay in a shared resource laboratory.

Morchauser, A, Yang, Z, Murphy, W, Tian, S, Issadore, D, Moore, J., CytoVirtual Interactive 2021, 2021

Development of a 35-color high parameter immune monitoring panel using a novel spectral cytometer.

Jones,D, Schretzenmair, RD, Jakobowski, J, Brake-Silla, N, Peterson, T, Steichen, A, Wu, A, Balderas, R, Moore, J., CytoVirtual Interactive 2021, 2021

HitMapper: High throughput mapper for relational analysis of cell states.

Ionita, M, Rogers, W, Schretzenmair, R, Jones, D, Greenplate, A, Moore, J, Wang, L-S., CytoVirtual Interactive 2021, 2021

Circulating donor lung-specific exosome profiles enable noninvasive monitoring of acute rejection in a rodent orthotopic lung transplantation model.

Habertheuer, Andreas MD, PhD1,2; Ram, Chirag BA1; Schmierer, Maggie MS3; Chatterjee, Shampa PhD4; Hu, Robert BA1; Freas, Andrew1; Zielinski, Patrick MS1; Rogers, Wade PhD3,5; Silvestro, Eva M. MLT, ASCP3,5; McGrane, Michael BS3; Moore, Jonni S. PhD5; Korutla, Laxminarayana PhD1,6; Siddiqui, Sarmad PhD,7; Xin, Yi PhD7; Rizi, Rahim MS, PhD7; Tao, Jian Qin MD4; Kreisel, Daniel MD, PhD8; Naji, Ali MD, PhD9; Ochiya, Takahiro PhD10; Vallabhajosyula, Prashanth MD, MS, Transplantation, 2021

PanoplyCF: A New R Package for Manifold Learning Clustering via Binning.

Rogers W, Ionita M, Moore J, CYTO Virtual 2020, 2020

Tailor: Targeting heavy tails in flow cytometry data with fast mixture modeling

Ionita M, Jones D, Moore J, Schretenmair R, Wang L-S, Rogers W., CYTO Virtual 2020, 2020

Fluster: A New R Package for Fingerprint-based Clustering

Rogers W, Ionita M, Moore J., CYTO Virtual 2020, 2020

FACSymphony A5 Harmonization and Validation for Computational Population Discovery

Schretzenmair RD, Jones DD, Brake-Silla G, Balderas R, Stall A, Moore JS, CYTO Virtual 2020, 2020

Robust Measurement of CD279 on Patients Receiving anti-PD-1 Therapy

Schretzenmair RD, Brake-Silla G, Jakubowski J, Jones DD, Moore JS., CYTO Virtual 2020, 2020

Development of a Flow Cytometry-Based Assay for Clinical Detection and Molecular Characterization of Breast Cancer Disseminated Tumor Cells in Bone Marrow

Chislock E, Woodfield G, Graves J, Pan T-C, Pant D, Chen Y, Shih N, Bayne L, Blanchard T, Smith M, Murphy W, Morchauser A, DeMuth W, Pletcher C, Moore J, Yu L, Bornheimer S, , Feldman M, Nayak A, DeMichele A, Chodosh L., CYTO Virtual 2020, 2020

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