Jonni S. Moore, PhD

Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
University of Pennsylvania Perelman School of Medicine
Consultant, Flow Cytometry

Contact Information211 and 297 John Morgan Building
3620 Hamilton Walk
Philadelphia, PA, 19104
Office: (215) 898-6853
Fax: (215) 898-4227


Specialty Division

Laboratory Medicine, Hematopathology, Cancer and Immunobiology

Research Expertise

Research Summary

Research in our laboratory centers on technology development with a focus on complex technologies, in particular cytomics. We pioneered new technology in flow cytometry liquid biopsies including the detection of extracellular vesicles as a rich source of prognostic and diagnostic information and developing this technology for diagnostics/prognostics in cardiovascular disease, traumatic brian injury and relapsing breast cancer. Most recently we have focused on the development of high dimensional deep phenotyping approaches for the identification of biomarkers of cancer responses using advance cytomic technology.
Current collaborators:
Lewis Chodosh, MD, PhD, Cancer Biology
Erica Carpenter, MBA, PhD, Department of Medicine
John Wherry, PhD, Department of Microbiology, Parker Institute for Cancer Immunotherapy

Flow Cytometry Expertise

As Director of the Penn Cytomics and Cell Sorting Shared Resource and founder of the Hospital of the University of Pennsylvania Clinical Flow Cytometry Laboratory as well as in my own research, I have developed and applied a variety of sophisticated flow cytometric applications with focus on translational application and computational interpretation. This includes expertise in intracellular protein detection, complex data mining, cell proliferation, rare stem cell detection as well as other functional assays. My lab has been recognized as "exceptional" by the NCI for over 30 years, and has been designated an International Society for the Advancement of Cytometry Recognized Shared Resource Laboratory in 2023.

Graduate Groups



B.A. (Biology), University of Virginia, Charlottesville, 1972
Ph.D. (Microbiology), Thomas Jefferson University, Philadelphia, 1984

Specialty Certification

Becton-Dickinson FACScan Key Operator, 1990
Becton-Dickinson FACStar PLUS Key Operator, 1992

Postgraduate Training

Postdoctoral Fellow, Immunology Graduate Group, Dr. Peter C. Nowell and Dr. Richard G. Hoover, University of Pennsylvania School of Medicine, 1984-1988

Awards and Honors

Keynote Speaker Award, National Flow Cytometry Annual Course, Bowdoin College, Maine, 212
Sigma Xi Research Society (Associate Member), 1981
Sigma Xi Student Research Award, 1983
Thomas Jefferson University Fellow, 1983-1984
National Institutes of Health Postdoctoral Fellow, 1984-1987
Chapter Scholar, Arthritis Foundation, 1987, 1988, 1990, 1987-1990
Academy of Clinical Laboratory Physicians and Scientists, 2000-present
Peter C. Nowell Teaching Award, 2002
FOCUS Award for the Advancement of Women in Medicine, 2008
College of American Pathologists Outstanding Inspector, 2008
David B.P. Goodman, M.D., Ph.D. Award
Department of Pathology and Laboratory Medicine, 2009
Cyto2011 Pre-Congress Scientific Mentor, International Society for the Advancement of Cytometry, 2011
The Carleton and Sigrid Stewart Lectureship, Great Lakes International Imaging and Fluorescence Association, 2012
Wallace H. Coulter Award for Lifetime Achievement for Contributions to the Science, Education and Practice of Clinical Cytometry
International Clinical Cytometry Society, 2016
Goodman Award for University Service, Department of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania, 2018
ISAC 2018 Membership Award for Advancing the Field of Cytometry, International Society for the Advancement of Cytometry, 2018
EXCEPTIONAL SHARED RESOURCE designation by NCI in Abramson Cancer Center comprehensive cancer center competitive renewal, 2020-2025
ISAC Leadership Award, International Society for the Advancement of Cytometry, 2022-2023
ISAC Recognized Shared Resource Laboratory, 2023
Innovation Day Scientist in the Spotlight
Slingshot Biosciences, 2023-2023

Memberships and Professional Organizations

American Association of Immunologists, 1985 - present
Penn Professional Women, 1991 - 2010
International Society for Advancement of Cytometry, 1992 - Present
Great Lakes International Imaging and Flow Cytometry Association, 1996 - Present
International Clinical Cytometry Society, 1996 - Present
National Institutes of Health Special Emphasis Panel (SBIR), Hematology 1,2 Study Section, 1998 - 2005
International Society for Hematopathology and Graft Engineering, 1999 - 2019
University of Rochester School of Medicine, 2003 - 2003
Center for Human Cell Therapy, CBR Institute for Biomedical Research, Harvard University Medical School, 2004 - 2007
University of California at San Diego, Department of Pathology and Cancer Center, 2004 - 2004
College of Graduate Studies, Thomas Jefferson University, 2005 - present
University of Alabama at Birmingham, Arthritis and Musculoskelatal Disease Center, 2005 - 2005
University of Michigan, Department of Pathology, 2006 - 2006
American Society for Investigative Pathology, 2006 - 2019
New York University Grossman School of Medicine, Flow Cytometry and Cell Sorting Shared Resource, 2011 - present
Association of Biomolecular Resource Facilities, 2015 - Present
National Cancer Institute, NIH, 2021 - 2021
Immune Health Institute Council member, Perelman School of Medicine, 2021 - present
National Cancer Institute, 2022 - 2022

Web Links

Selected Publications

Transcriptional profiling of single tumor cells from pleural effusions reveals heterogeneity of epithelial to mesenchymal transition and extra-cellular matrix marker expression

Moen Sen, 1 Ryan M. Hausler, 1 Keely Dulmage, 2 Taylor A. Black, 1 William Murphy, 3 Charles H. Pletcher Jr, 3Ling Wang, 2 Chang Chen, 2 Stephanie S. Yee, 1 Scott J. Bornheimer, 4 Kara N. Maxwell, 1 Ben Z. Stanger, 5Jonni S. Moore, 3 Jeffrey C. Thompson, 6 and Erica L. Carpenter 1, Clin Trans Med 12(7): , 2022, PMID:35811459

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555 Clinical and pharmacodynamic biomarker results from PORTER, a multi-cohort phase 1 platform trial of combination immunotherapy for metastatic castration-resistant cancer patients

Matthew Galsky, Karen Autio, Kristopher Wentzel, Julie Graff, Terence Friedlander, Julie Densmore,Christopher Cabanski, Kristin Shotts, Tim Howes, Jia Yu,Elaine Eisenbeisz, Marko Spasic,Stephen Maddock6 Diane DaSilva,Dinesh Kumar, Jonni Moore, Richard Schretzenmair, Jennifer Lata, Arron Xu, Emma Reuschel, Snehal Wani, Matthew Morrow, Jeffrey Skolnik, Sandra Santulli-Marotto, Lacey Padron, Lisa Butterfield, Theresa LaVallee, Samantha Bucktrout, Michael Yellin, Tibor Keler9 Lisa Salvador, Jill O'Donnell-Tourmey, Vanessa Lucey, Justin Fairchild, Ute Dugan, Nina Bhardwaj, Sumit Subudhi, Lawrence Fong, Journal for ImmunoTherapy of Cancer 10(): , 2022

Sotigalimab and/or nivolumab with chemotherapy in first-line metastatic pancreatic cancer: clinical and immunologic analyses from the randomized phase 2 PRINCE trial

PadrĂ³n LJ, Maurer DM, O'Hara MH, O'Reilly EM, Wolff RA, Wainberg ZA, Ko AH, Fisher G, Rahma O, Lyman JP, Cabanski CR, Yu JX, Pfeiffer SM, Spasic M, Xu J, Gherardini PF, Karakunnel J, Mick R, Alanio C, Byrne KT, Hollmann TJ, Moore JS, Jones DD, Tognetti M, Chen RO, Yang X, Salvador L, Wherry EJ, Dugan U, O'Donnell-Tormey J, Butterfield LH, Hubbard-Lucey VM, Ibrahim R, Fairchild J, Bucktrout S, LaVallee TM, Vonderheide RH., Nat.Med 28(): 1167, 2022, PMID:35662283

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Circulating Donor Lung-specific Exosome Profiles Enable Noninvasive Monitoring of Acute Rejection in a Rodent Orthotopic Lung Transplantation Model.

Habertheuer, Andreas; Ram, Chirag; Schmierer, Maggie; Chatterjee, Shampa; Hu, Robert; Freas, Andrew; Zielinski, Patrick; Rogers, Wade; Silvestro, Eva M; McGrane, Michael; Moore, Jonni S; Korutla, Laxminarayana; Siddiqui, Sarmad; Xin, Yi; Rizi, Rahim; Qin Tao, Jian; Kreisel, Daniel; Naji, Ali; Ochiya, Takahiro; Vallabhajosyula, Prashanth, Transplantation 106 (4): 754-766, 2022, PMID:33993180

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Howard M. Shapiro: Remembering Cytometry's Muse

Moore, J, Cytometry Part A 99(12): 1169, 2021, PMID:34851015

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Implementation of a single cell double-emulsion droplet assay in a shared resource laboratory.

Morchauser, A, Yang, Z, Murphy, W, Tian, S, Issadore, D, Moore, J., CytoVirtual Interactive 2021, 2021

Development of a 35-color high parameter immune monitoring panel using a novel spectral cytometer.

Jones,D, Schretzenmair, RD, Jakobowski, J, Brake-Silla, N, Peterson, T, Steichen, A, Wu, A, Balderas, R, Moore, J., CytoVirtual Interactive 2021, 2021

HitMapper: High throughput mapper for relational analysis of cell states.

Ionita, M, Rogers, W, Schretzenmair, R, Jones, D, Greenplate, A, Moore, J, Wang, L-S., CytoVirtual Interactive 2021, 2021

Reconcile the debate over protective effects of BCG vaccine against COVID-19.

Fu W, Ho PC, Liu CL, Tzeng KT, Nayeem N, Moore JS, Wang LS, Chou SY, Sci Rep 11(1): , 2021, PMID:33863950

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Tailor: Targeting heavy tails in flow cytometry data with fast mixture modeling

Ionita M, Jones D, Moore J, Schretenmair R, Wang L-S, Rogers W., Cytometry A 99(2): 133-144, 2021, PMID:33476090

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