Jonni S. Moore, PhD
Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania
University of Pennsylvania Perelman School of Medicine
Consultant, Flow Cytometry
Contact Information211 and 297 John Morgan Building
3620 Hamilton Walk
Philadelphia, PA, 19104
Office: (215) 898-6853
Fax: (215) 898-4227
Email: moorej@pennmedicine.upenn.edu
Specialty Division
Laboratory Medicine, Hematopathology, Cancer and Immunobiology
Research Expertise
Research Summary
Research in our laboratory centers on technology development with a focus on complex technologies, in particular cytomics. We pioneered new technology in flow cytometry liquid biopsies including the detection of extracellular vesicles as a rich source of prognostic and diagnostic information and developing this technology for diagnostics/prognostics in cardiovascular disease, traumatic brian injury and relapsing breast cancer. Most recently we have focused on the development of high dimensional deep phenotyping approaches for the identification of biomarkers of cancer responses using advance cytomic technology.
Current collaborators:
Lewis Chodosh, MD, PhD, Cancer Biology
Erica Carpenter, MBA, PhD, Department of Medicine
John Wherry, PhD, Department of Microbiology, Parker Institute for Cancer Immunotherapy
Flow Cytometry Expertise
As Director of the Penn Cytomics and Cell Sorting Shared Resource and founder of the Hospital of the University of Pennsylvania Clinical Flow Cytometry Laboratory as well as in my own research, I have developed and applied a variety of sophisticated flow cytometric applications with focus on translational application and computational interpretation. This includes expertise in intracellular protein detection, complex data mining, cell proliferation, rare stem cell detection as well as other functional assays. My lab has been recognized as "exceptional" by the NCI for over 30 years, and has been designated an International Society for the Advancement of Cytometry Recognized Shared Resource Laboratory in 2023. In the last decade, I have led a significant movement in the shared resource laboratory domain to create the concept of Academic Enterprise as the basis of successful shared resource laboratories.
Graduate Groups
Immunology
Education
B.A. (Biology), University of Virginia, Charlottesville, 1972
Ph.D. (Microbiology), Thomas Jefferson University, Philadelphia, 1984
Specialty Certification
Becton-Dickinson FACScan Key Operator, 1990
Becton-Dickinson FACStar PLUS Key Operator, 1992
Postgraduate Training
Postdoctoral Fellow, Immunology Graduate Group, Dr. Peter C. Nowell and Dr. Richard G. Hoover, University of Pennsylvania School of Medicine, 1984-1988
Awards and Honors
Keynote Speaker Award, National Flow Cytometry Annual Course, Bowdoin College, Maine, 212
Sigma Xi Research Society (Associate Member), 1981
Sigma Xi Student Research Award, 1983
Thomas Jefferson University Fellow, 1983-1984
National Institutes of Health Postdoctoral Fellow, 1984-1987
Chapter Scholar, Arthritis Foundation, 1987, 1988, 1990, 1987-1990
Academy of Clinical Laboratory Physicians and Scientists, 2000-present
Peter C. Nowell Teaching Award, 2002
FOCUS Award for the Advancement of Women in Medicine, 2008
College of American Pathologists Outstanding Inspector, 2008
David B.P. Goodman, M.D., Ph.D. Award
Department of Pathology and Laboratory Medicine, 2009
Cyto2011 Pre-Congress Scientific Mentor, International Society for the Advancement of Cytometry, 2011
The Carleton and Sigrid Stewart Lectureship, Great Lakes International Imaging and Fluorescence Association, 2012
Wallace H. Coulter Award for Lifetime Achievement for Contributions to the Science, Education and Practice of Clinical Cytometry
International Clinical Cytometry Society, 2016
Elected to Executive Committee, President-Elect, then President of the International Society for the Advancement of Cytometry, 2018-2026
ISAC 2018 Membership Award for Advancing the Field of Cytometry, International Society for the Advancement of Cytometry, 2018
Goodman Award for University Service, Department of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania, 2018
EXCEPTIONAL SHARED RESOURCE designation by NCI in Abramson Cancer Center comprehensive cancer center competitive renewal, 2020-2025
ISAC Leadership Award, International Society for the Advancement of Cytometry, 2022-2023
Innovation Day Scientist in the Spotlight
Slingshot Biosciences, 2023-2023
ISAC Recognized Shared Resource Laboratory, 2023
Memberships and Professional Organizations
American Association of Immunologists, 1985 - present
Penn Professional Women, 1991 - 2010
International Society for Advancement of Cytometry, 1992 - Present
International Clinical Cytometry Society, 1996 - Present
Great Lakes International Imaging and Flow Cytometry Association, 1996 - Present
National Institutes of Health Special Emphasis Panel (SBIR), Hematology 1,2 Study Section, 1998 - 2005
International Society for Hematopathology and Graft Engineering, 1999 - 2019
University of Rochester School of Medicine, 2003 - 2003
University of California at San Diego, Department of Pathology and Cancer Center, 2004 - 2004
Center for Human Cell Therapy, CBR Institute for Biomedical Research, Harvard University Medical School, 2004 - 2007
University of Alabama at Birmingham, Arthritis and Musculoskelatal Disease Center, 2005 - 2005
College of Graduate Studies, Thomas Jefferson University, 2005 - present
University of Michigan, Department of Pathology, 2006 - 2006
American Society for Investigative Pathology, 2006 - 2019
New York University Grossman School of Medicine, Flow Cytometry and Cell Sorting Shared Resource, 2011 - present
Association of Biomolecular Resource Facilities, member, 2015 - Present
Immune Health Institute Council member, Perelman School of Medicine, 2021 - present
National Cancer Institute,P30 IAR for P30 for Dana Farber Comprehensive Cancer Center at Harvard University., 2021 - 2021
National Cancer Institute, P30 IAR Reviewer for Massey Cancer Center for on site review October, 2022, 2022 - 2022
Web Links
Selected Publications
Evaluation of the Imaging Capabilities of the Discover A8
Derek D. Jones1, Jennifer Jakubowski1, William Murphy1, and Jonni S. Moore1, International Society for the Advancement of Cytometry 2026, Abstract,, 2026
Clinical and translational results from a phase 1 trial of gemcitabine/nab-paclitaxel with nivolumab/ipilimumab or hydroxychloroquine/ipilimumab in untreated metastatic pancreatic adenocarcinoma.
O'Reilly EM, Cabanski CR, Lyman JP, Wainberg ZA, Fisher GA, Wolff RA, Ko AH, O'Hara MH, Spencer CN, Yu JX, Da Silva DM, Padrón LJ, Arnott J, Fairchild J, Moore JS, Peng B, Hoos WA, O'Donnell-Tormey J, Boffo S, Dugan U, Kimmelman AC, Amaravadi RK, Vonderheide RH., J Immunother Cancer Jan 30;14(1):e012864(): , 2026
DTC-Flow: a flow cytometry-based detection platform for characterizing bone marrow disseminated tumor cells in breast cancer.
Chislock EM, Pan TC, Pant D, Chen Y, Woodfield G, Graves J, Lawrence-Paul MR, Belka GK, Wang J, Bayne L, Blanchard T, Smith M, Ji X, Shih NNC, Soucier-Ernst D, Goodman N, Nivar I, Goodspeed B, DeLuca S, Ndicu J, Clark C, Langer M, William Stavropoulos S, DeMuth W, Morschauser A, Murphy W, Nayak A, Feldman M, Clark A, Yu L, Judge K, Bornheimer S, Pletcher CH Jr, Moore JS, DeMichele A, Chodosh, NPJ Breast Cancer. 2025 Nov 24;11(1):139(): , 2025
Evaluating Micro-5 as a Biosafety Alternative to Cyclex-D in Novel Aerosol Containment Testing
Shifu Tian, Charles H. Pletcher Jr., Hongen Wang, William Murphy, Jonni S. Moore, ISAC Annual Meeting, Cyto 2025 Denver, 2025
How to Build Effective Training Modalities in a Shared Resource Laboratory
Jennifer Jakubowski, William Murphy, Derek D. Jones, Jacquelyn Thomas, Jonni S. Moore ,Department of Pathology and Laboratory Medicine, Abramson Cancer Center, Penn Cytomics and Cell Sorting Resource Laboratory, Perelman School of Medicine of the University of Pennsylvania, Philadelphia, PA, CYTO 2025 annual meeting of ISAC-Denver, 2025
Impact of Instrument Configuration and Compensation Methods on the Performance of a Large Library of Fluorochromes: Critical Concerns for Panel and Assay Design
William Murphy1, Richard Schretzenmair1, Samantha Pearson1, Jennifer Jakubowski1, Derek Jones1, Robert Balderas2, Jonni Moore1 1University of Pennsylvania, Penn Cytomics and Cell Sorting Shared Resource Laboratory, Philadelphia, PA 2BD Biosciences, Sane Jose, CA, Annual meeting of ISAC, CYTO 2025, 2025
When the Powerhouse Powers Down: Development of a Functional Flow Cytometric Panel to Identify Changes in Mitochondrial Phenotypes During Induced Cell Death Pathways
Pearson, S., Schretzenmair, R., Moore, J., Jones, D., Annual meeting of ISAC, CYTO 2025 Denver, 2025
Immunologic signatures of response and resistance to nivolumab with ipilimumab in advanced metastatic cancer.
Apostolia M. Tsimberidou, Farah A. Alayli, Kwame Okrah, Alexandra Drakaki, Danny N. Khalil, Shivaani Kummar, Saad A. Khan, F. Stephen Hodi, David Y. Oh, Christopher R. Cabanski, Shikha Gautam, Stefanie L. Meier, Meelad Amouzgar, Shannon M. Pfeiffer, Robin Kageyama, EnJun Yang, Marko Spasic, Michael T. Tetzlaff, Wai Chin Foo, Travis J. Hollmann, Yanyun Li, Matthew Adamow, Phillip Wong, Jonni S. Moore, Sharlene Velichko, Richard O. Chen, Dinesh Kumar, Samantha Bucktrout, Ramy Ibrahim, Ute Dugan, Lisa Salvador, Vanessa M. Hubbard-Lucey, Jill O’Donnell-Tormey, Sandra Santulli-Marotto, Lisa H. Butterfield, Diane M. Da Silva, Justin Fairchild, Theresa M. LaVallee, Lacey J. Padrón, Padmanee Sharma, . J Exp Med 221(): 221 (10), 2024
Metabolite patterns associated with individual response to supervised exercise therapy in patients with intermittent claudication
Bellomo TR, Tsao NL, Johnston-Cox H, Borkowski K, Shakt G, Judy R, Moore J, Ractcliffe SJ, Fiehn O, Floyd TF, Wehrli FW, Mohler E, Newman JW, Damrauer SM., JVS Vasc Sci. 3(): 379-388, 2022, PMID:36568282
Transcriptional profiling of single tumor cells from pleural effusions reveals heterogeneity of epithelial to mesenchymal transition and extra-cellular matrix marker expression
Moen Sen, 1 Ryan M. Hausler, 1 Keely Dulmage, 2 Taylor A. Black, 1 William Murphy, 3 Charles H. Pletcher Jr, 3Ling Wang, 2 Chang Chen, 2 Stephanie S. Yee, 1 Scott J. Bornheimer, 4 Kara N. Maxwell, 1 Ben Z. Stanger, 5Jonni S. Moore, 3 Jeffrey C. Thompson, 6 and Erica L. Carpenter 1, Clin Trans Med 12(7): , 2022, PMID:35811459
